Effects of the Cell Cycle Inhibitor Olomoucine on Inflammatory Response and Neuronal Cell Death after Spinal Cord Injury in Rats  

作　　者：TIAN Dai-shi XIE Min-jie YU Zhi-yuan ZHANG Qiang WANG Yi-hui CHEN Bin CHEN Chen WANG Wei 

机构地区：[1]Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

出　　处：《神经损伤与功能重建》2007年第3期143-151,共9页Neural Injury and Functional Reconstruction

基　　金：the National Science Foundation of China(C30230140)

摘　　要：Objective:The influence of olomoucine on microglial proliferation with associated inflammatory response after spinal cord injury has been determined.Methods:Microglial proliferation and neuronal apoptosis were observed by immunofluorescence.Level of the proinflammatory cytokine interleukin-1β(IL-1β)expression in the injured cord was determined by Western blot analysis.Results:the cell cycle inhibitor olomoucine,administered at 1 h post injury,significantly suppressed microglial proliferation and produced a remarkable reduction of tissue edema formation.In the olomoucine-treated group,a significant reduction of activated and/or proliferated microglial induced IL-1β expression was observed 24 h after SCI.Moreover,olomoucine evidently attenuated the number of apoptotic neurons after SCI.Conclusion:Our findings suggest that modulation of microglial proliferation with associated proinflammatory cytokine expression may be a mechanism of cell cycle inhibition-mediated neuroprotections in the CNS trauma.Objective： The influence of olomoucine on microglial proliferation with associated inflammatory response after spinal cord injury has been determined. Methods： Microglial proliferation and neuronal apoptosis were observed by immunofluorescence. Level of the proinflammatory cytokine interleukin-1β （IL-1β） expression in the injured cord was determined by Western blot analysis. Results： the cell cycle inhibitor olomoucine, administered at 1 h post injury, significantly suppressed microglial proliferation and produced a remarkable reduction of tissue edema formation. In the olomoucine-treated group, a significant reduction of activated and/or proliferated microglial induced IL-1β expression was observed 24 h after SCI. Moreover, olomoucine evidently attenuated the number of apoptotic neurons after SCI. Conclusion：Our findings suggest that modulation of microglial proliferation with associated proinflammatory cytokine expression may be a mechanism of cell cycle inhibition-mediated neuroprotections in the CNS trauma.

关 键 词：脊髓损伤 鼠 细胞循环抑制剂 神经胶质细胞 神经元 细胞凋亡 炎症反应 

分 类 号：R744[医药卫生—神经病学与精神病学]