海带多糖对肾上腺素致血管内皮细胞损伤的防护作用  

THE PROTECTIVE EFFECT OF POLYSACCHARIDE EXTRACTED FROM LAMINARIA JAPONICA ARESCH ON VESSES ENDOTHELIAL CELL INJURY INDUCING BY ADRENALINE

作　　者：谢露[1] 刘爱群[2] 黎静[1] 陈蒙华[3] 

机构地区：[1]广西医科大学生理学教研室,广西南宁530021 [2]广西医科大学肿瘤医院,广西南宁530027 [3]广西医科大学附属医院心血管病研究所,广西南宁530027

出　　处：《中国应用生理学杂志》2007年第2期143-147,共5页Chinese Journal of Applied Physiology

基　　金：广西自然科学资金资助课题(0229033);广西卫生厅课题(Z2006057)

摘　　要：目的探讨海带多糖L01对肾上腺素(Adr)致血管内皮细胞(VEC)损伤的保护作用。方法采用注射Adr法建立VEC损伤大鼠模型,主动脉切片免疫组化检测血管内皮受损情况,ELISA法测定大鼠血浆血管性血友病因子(von Willebrand factor,vWF)含量;体外培养人脐静脉内皮细胞(HUVEC),ELISA法测定HUVEC培养液vWF含量,观察L01对VEC损伤大鼠和Adr刺激HUVEC后vWF生成的影响。结果造模第4d和第5d主动脉切片免疫组化检测完整内皮层长度(μm)显示,L01高剂量(50mg/kg)、低剂量(10mg/kg)组长度明显高于模型组(P<0.05);造模第4d,L01高剂量组大鼠血浆vWF水平低于模型组,两者比较有显著性差异(P<0.05),第5dL01高、低剂量组大鼠血浆vWF水平均低于模型组(P<0.05)。在HUVEC培养实验中,终浓度为0.01mg/ml和0.1mg/ml的L01均能降低24h培养液vWF水平,终浓度为0.1mg/mlL01还能降低48h培养液vWF水平,与Adr组比较有显著性差异(P<0.05)。结论L01对VEC具有保护作用。To study the protective effect of Polysaccharide of Laminaria LO1 on endothelial cell injury inducing by adrenaline. Methods： In order to observe the influence of LO1 on the release of vWF in endothelial injured rats and HUVEC stimulated by adrenaline, a rat model of endothelial injury was established via injecting adrenaline, the damaged degree of vascular endothelial was evaluated by aortic immunity histochemistry, HUVEC was cultured in vitro, the content of vWF in rat plasma and in supernatant was measured by ELISA. Results： The measure of intact endodermis lengths（/im）stained by immunohistochemistry demonstrated the length in L01 high-dose group and low-dose group was obviously longer than that of model group（ P 〈 0.05 ） in the 4th and 5th day during the modal made. The content of vWF in rat plasma of L01 high-dose group was lower than that of model group（P〈0.05） in the 4th day, there were significant differences between this two groups, and the content of vWF in rat plasma of both L01 high-dose group and low-dose group was lower than that of model group（P〈0.05） in the 4th and 5th days. In the study of cultured HUVEC, on the 24 h, L01 groups（0.01 mg/ml and 0.1 mg/ml） decreased the supernatant vWF levd, and on the 48 h, high-dose group（0.1 mg/ml） also decreased the supernatant vWF level, with significant difference compared with adrenaline group（ 10 μg/ml, P 〈 0.05 ）. Conclusion： L01 presented the protective effect on vascular endothelial cell.

关 键 词：海带 多糖 内皮细胞 血管性血友病因子(von Willebrand FACTOR vwF) 肾上腺素 

分 类 号：R151[医药卫生—营养与食品卫生学] R331.3[医药卫生—公共卫生与预防医学]