马尾松枝条挥发性组分的鉴定及松墨天牛对其触角电生理反应  

Volatiles from wattles of Pinus massoniana and EAG responses of the adult Monochamus alternatus to the identified chemicals

作　　者：李水清[1] 张钟宁[1] 

机构地区：[1]中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室

出　　处：《昆虫知识》2007年第3期385-389,共5页Entomological Knowledge

基　　金：中国科学院知识创新工程重大项目(KSCX1-SW-13)

摘　　要：为研究松墨天牛Monochamus alternatus Hope取食期引诱剂,利用气相色谱-质谱(GC-MS)联用技术并与标样核对分析了马尾松(Pinus massoniana Lamb.)枝条挥发性气味的化学组成。结果表明,马尾松1年生、2年生、3年生枝条气味均由8种萜烯类物质组成,但各组分相对含量不同。经方差分析,α-蒎烯、莰烯、β-蒎烯、柠檬烯、萜品油烯、β-石竹烯在3种枝条中的相对百分含量的差异均达到极显著水平。1年生枝条中α-蒎烯、莰烯和萜品油烯的相对含量最小,分别为20.81%、0.43%和4.74%,而β-蒎烯、月桂烯和柠檬烯的相对含量最高,分别为12.37%、4.33%和4.53%。鉴定的化合物对松墨天牛雌雄成虫的触角电生理反应的实验,结果表明,松墨天牛雌雄成虫对α-蒎烯的反应最强,对β-石竹烯的反应最弱。The volatiles from wattles of Pinus rnassoniana Lamb. were determined by gas chromatography-mass spectrometry （GC-MS）. The results showed that there were eight constituents in volatiles of one-, two- and three-yearold wattles of P. massoniana, which were consisted of α-pinene, camphene, β-pinene, myrcene, limonene, β- phellandrene, terpinolene and β-caryophyllene. But relative contents of these chemicals varied with wattle ages, with considerable difference in α-pinene, camphene, β-pinene, limonene, terpinolene and β-caryophyllene. The relative contents of α-pinene, camphene and terpinolene in one-year-old wattles were the least, which was 20.81%, 0.43 % and 4.74% respectively. Whereas, the relative contents of β-pinene, myrcene and limonene were the most, which was 12.37%, 4.33% and 4.53% respectively. Electroantennogram （EAG） responses of the adult Monochamus alternatus Hope to these identified chemicals were tested and α-pinene caused maximum EAG responses, and β- caryophyllene the least.

关 键 词：气相色谱-质谱(GC-MS) 挥发物鉴定 马尾松 萜类 质谱学 松墨天牛 触角电位 

分 类 号：S763.7[农业科学—森林保护学]