炎症性肠病患者外周血单个核细胞细胞毒性T淋巴细胞相关抗原4的表达  

Cytotoxic T Lymphocyte-associated Antigen 4 Expression on Surface of Peripheral Blood Mononuclear Cells in Patients with Inflammatory Bowel Disease

作　　者：饶玉霞[1] 袁岸龙[1] 李春[1] 叶梅[1] 李瑾[1] 姜黎[1] 夏冰[1] 

机构地区：[1]武汉大学中南医院消化系病研究中心,430071

出　　处：《胃肠病学》2007年第5期270-273,共4页Chinese Journal of Gastroenterology

基　　金：国家自然科学基金项目(No.30470783)

摘　　要：背景:细胞毒性T淋巴细胞相关抗原(CTLA)4是一种重要的免疫细胞共刺激分子,主要表达于活化的T淋巴细胞,对T细胞的激活起抑制作用。目的:通过检测炎症性肠病(IBD)患者外周血单个核细胞表面CTLA4的表达,探讨CTLA4在IBD发病中的作用和功能。方法:将18例活动性溃疡性结肠炎(UC)、4例活动性克罗恩病(CD)患者以及21名健康对照者外周血单个核细胞分离后,以流式细胞仪检测细胞表面CTLA4、CD4+和CD8+的表达。结果:自然状态下,UC和CD患者CD4+CTLA4+表达显著高于对照组(8.2%±4.7%对1.4%±1.3%,P<0.001;4.3%±2.9%对1.4%±1.3%,P=0.011);CD8+CTLA4+表达亦显著高于对照组(9.1%±7.2%对0.9%±0.3%;8.7%±3.5%对0.9%±0.3%,P均<0.001)。经植物血凝素(PHA)刺激后,IBD患者和对照组外周血单个核细胞CTLA4的表达均增加;CD组CD8+CTLA4+表达显著高于对照组(35.0%±10.9%对18.1%±10.1%,P=0.005)。结论:活动性UC和CD患者外周血单个核细胞CTLA4表达增强,提示T细胞激活第二信号通路B7/CD28/CTLA4在IBD的发病过程中起重要作用。Background： Cytotoxic T lymphocyte-associated antigen （CTLA） 4 is an important co-stimulating factor for immune cells, which is expressed on the surface of activated T cells and plays an inhibiting role in T cell activation. Ahns： To investigate the expression of CTLA4 on surface of peripheral blood mononuclear cells in inflammatory bowel disease （IBD） and to explore its role in the pathogenesis of IBD. Methods： Peripheral blood mononuclear cells were isolated from 18 patients with active ulcerative colitis （UC）, 4 with active Crohn＇s disease （CD） and 21 healthy controls. CTLA4, CD4^＋ and CD8^＋ expression on the cells was determined by flow cytometry. Results： Spontaneous CTLA4 expression on CD4^＋ and CD8^＋ cells in patients with UC and CD were significantly higher than those in healthy controls （CD4^＋ cells： 8.2%±4.7% vs. 1.4%±1.3%, P〈0.001 in UC, and 4.3%±2.9% vs. 1.4%±1.3%, P=0.011 in CD, respectively; CD8^＋ cells： 9.1%±7.2% vs. 0.9%±0.3%, P〈0.001 in UC, and 8.7%±3.5% vs. 0.9%±0.3%, P〈0.001 in CD, respectively）. After the cells were stimulated with mitogen phytohemagglutinin, the expression of CTLA4 increased markedly in both IBD patients and healthy controls to a similar magnitude, and only the expression of CTLA4 on CD8^＋ cells in patients with CD was still higher than that in healthy controls （35.0%±10.9% vs. 18.1%±10.1%, P=0.005）. Conclusions： CTLA4 expression is upregulated in patients with active UC and CD, which suggests that T cell activation by the second signal of B7/CD28/ CTLA4 plays important role in the pathogenesis of IBD.

关 键 词：结肠炎 溃疡性 CROHN病 细胞毒性T淋巴细胞相关抗原4 外周血单个核细胞 流式细胞术 

分 类 号：R574[医药卫生—消化系统]