c-erbB-2、bcl-2和p53在结直肠肿瘤中的表达及其临床意义  

Expression and Clinical Significances of c-erbB-2,bcl-2 and p53 in Colorectal Neoplasms

作　　者：薄挽澜[1] 胡凤丽[2] 翟博[3] 姜爱民[1] 

机构地区：[1]哈尔滨医科大学附属第一医院消化内科,150001 [2]哈尔滨市第一医院消化内科 [3]哈尔滨医科大学附属第四医院普外科

出　　处：《胃肠病学》2007年第5期279-282,共4页Chinese Journal of Gastroenterology

摘　　要：背景:c-erbB-2、bcl-2和突变型p53在结直肠腺瘤癌变过程中相互调节并发挥重要作用。目的:探讨结直肠肿瘤中c-erbB-2、bcl-2和p53蛋白的表达及其临床意义。方法:取42例结直肠腺癌、10例腺瘤和10例正常结直肠黏膜组织,以免疫组化方法检测其中c-erbB-2、bcl-2和p53蛋白的表达,分析其表达与腺癌临床病理特征的关系。结果:c-erbB-2、bcl-2和p53蛋白在腺癌、腺瘤和正常黏膜组织中的表达差异均有统计学意义(P<0.05),bcl-2蛋白在腺癌组织中的表达低于腺瘤组织,c-erbB-2和p53蛋白在腺癌组织中的表达高于腺瘤和正常黏膜组织。c-erbB-2蛋白的表达随腺癌分化程度的降低而增高,bcl-2蛋白的表达随腺癌分化程度的降低而降低;c-erbB-2和p53蛋白的表达与淋巴结转移和Dukes分期呈正相关。腺癌组织中bcl-2与p53蛋白的表达呈负相关(rs=-0.301,P<0.05)。结论:c-erbB-2与结直肠癌的进展、分化和转移相关。腺癌组织中p53高表达和bcl-2相对低表达提示两者可能参与了结直肠癌的发生、发展过程,bcl-2过表达可能在结直肠癌发生的早期起作用。Background： c-erbB-2, bcl-2 and mutation of p53 are mutually interregnlated and may play important roles in the carcinomatous changes of colorectal adenoma. Aims： To appraise the protein expression of c-erbB-2, bcl-2 and p53 in colorectal neoplasms and their clinical significances. Methods： lmmunohistochemical method was used to detect the protein expression of c-erbB-2, bcl-2 and p53 in 42 colorectal adenocarcinoma, 10 adenoma and 10 normal colorectal mucosal tissue. The relationships between these protein expression and the clinicopathologic characteristics of colorectal adenoeareinoma were analyzed. Results： Significant differences could be found among colorectal adenoearcinoma, adenoma and normal colorectal mucosal tissue in the protein expression of c-erbB-2, bcl-2 and p53 （P〈0.05）. bcl-2 protein expression in colorectal adenocarcinoma was decreased as compared with that in the adenoma while the protein expression of c-erbB-2 and p53 in colorectal adenocarcinoma was increased. From well-, moderately- to poorly-differentiated adenoearcinoma of colorectum, the protein expression of c-erbB-2 increased and bcl-2 decreased gradually, and protein expression of c-erbB-2 and p53 was positively correlated with lymph node metastasis and Dukes staging of colorectal adenocarcinoma. Negative correlation was found between the protein expression of bcl-2 and p53 in the colorectal adenoeareinoma （rs=-0.301, P〈0.05）. Conclusions： c-erbB-2 is involved in the progression, differentiation and metastasis of colorectal cancer. High expression of p53 and relatively low expression of bcl-2 may be correlated with the occurrence and development of eolorectal cancer. Over-expression of bcl-2 may be an early event in the development of colorectal cancer.

关 键 词：结直肠肿瘤 基因 ERBB-2 基因 BCL-2 基因 p53 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]