孔雀石绿对三种鱼类细胞急性毒性的初步研究  

Acute Toxicity of Malachite Green to Three Kinds of Fish Cell Line

作　　者：文琛[1] 邬红娟[1] 王宇飞[1] 

机构地区：[1]华中科技大学环境科学与工程学院,武汉430074

出　　处：《环境科学与技术》2007年第6期8-9,34,共3页Environmental Science & Technology

基　　金：农业部948课题资助(2005Z37)

摘　　要：应用MTT法测定孔雀石绿对鲤鱼上皮癌细胞(EPC)、草鱼肾细胞(CIK)、斑点叉尾鮰鱼卵巢细胞(CCO)的急性毒性。测定孔雀石绿的细胞毒性,发现MTT还原量减少,甲瓒生成量与孔雀石绿浓度存在依赖关系,孔雀石绿浓度越大,活细胞越少,甲瓒生成量也相应减少。以2×104接种量对EPC细胞、CIK细胞、CCO细胞进行24h染毒,发现随着孔雀石绿浓度的增高,细胞存活率减少,并确定孔雀石绿对这三种细胞的安全浓度、半致死浓度和致死浓度。比较这三种细胞对孔雀石绿的敏感性,得出结论CIK细胞对于孔雀石绿的作用最敏感。An attempt was made to collect information of toxicity of malachite green by MTT assay. EPC cell, CIK cell and CCO cell were exposed to malachite-green-polluted states with live cell numbers being measured by MTT assay. Result indicates that livability of EPC cell, CIK cell and CCO cell depends on concentration of malachite green after 24h being contaminated. Among the cells tested CIK is the most sensitive one to malachite green.

关 键 词：孔雀石绿 MTT法 鱼细胞 急性毒性 

分 类 号：X171.5[环境科学与工程—环境科学]