大孔载体制备及其固定化脂肪酶  

Preparation of macroporous carrier for immobilization of lipase

作　　者：蔡宏举[1] 王满意[1] 周鑫[1] 谭天伟[1] 

机构地区：[1]北京市生物加工过程重点实验室,北京化工大学生命科学与技术学院,北京100029

出　　处：《化工学报》2007年第6期1529-1534,共6页CIESC Journal

基　　金：国家自然科学基金项目(20636010;50373003;20406002);国家杰出青年基金项目(20325622);北京市科技计划项目(D0205004040211);北京化工大学青年教师基金(QN0616);国家高技术研究发展计划项目(2006AA020203)。~~

摘　　要：以甲基丙烯酸缩水甘油酯（GMA）为单体,二乙烯基苯（DVB）为交联剂,采用固液联合致孔的方式,通过本体聚合制备具有超大孔的含有环氧基团的多孔载体。其孔径分布为典型的双孔分布,其中150-400nm的孔区约占总孔容的60%,孔隙率为59.82%,比表面积29.60m2·g^-1。载体的环氧基团水解成羟基后,以戊二醛为偶联剂固定化脂肪酶。在相同条件下,比较了不同孔结构载体的固定化假丝酵母脂肪酶催化橄榄油水解的效果。结果表明,具有一定超大孔结构的多孔载体固定化酶的效果最好。同时考察了固定化脂肪酶在正己烷中催化十二酸辛醇的酯化反应活力。Macroporous poly （glycidyl methacrylate-co-divinylbenzene） carrier containing epoxide group was prepared by bulk polymerization using a multiple porogen consisting of nano-granules of calcium carbonate as solid porogen and the mixture of toluene and heptane as liquid porogen. The results revealed that the carrier, with the porosity of 59.82~ff0 and specific surface area of 29.60 m2· g^-1, mainly contained two kinds of pores, macropores （150-400 nm, approximately 60%） and superpores （2000--8000 nm, about 14. 9%）. The epoxide groups were hydrolyzed in H＋ atmosphere to afford hydroxyl group, which subsequently reacted with glutaraldehyde for the immobilization of lipase. The activities of lipase on different carriers were investigated by catalyzing hydrolysis of olive oil. The result showed that the immobilized lipase on the carrier with double pore structure showed the highest activity of catalyzing esterification of lauric acid. Furthermore, the esterification activity of immobilized lipase was also investigated by using the reaction of n-octanol and dodecanic acid in n-hexane.

关 键 词：大孔载体 固定化 脂肪酶 

分 类 号：Q814.2[生物学—生物工程]