荧光法猝灭测定木槿叶中混合氨基酸的总量  

Fluorescence quenching spectrophotometry for determination of mixed amino acids in hibiscus syriacus L.Leaves

作　　者：任凤莲[1] 肖劲[1] 廖律[1] 宋鸽[1] 

机构地区：[1]中南大学化学化工学院,长沙410083

出　　处：《分析试验室》2007年第6期73-76,共4页Chinese Journal of Analysis Laboratory

摘　　要：在乙酸-乙酸钠缓冲介质中,氨基酸能猝灭壳聚糖-茚三酮体系荧光,基于此,对木槿叶中混合氨基酸进行测定,并对该方法的测定条件进行了讨论.线性范围为0.5～3.0 mmol/L,检出限为0.1 mmol/L.木槿叶干粉中游离氨基酸的质量分数为10.18%,相对标准偏差为1.3%,回收率为102.5%～94.3%.The mixed amino acids from Hibiscus syriacus L. leaves have been determined, which was based on fluorescence quenching effect by adding amino acid into the reaction system of chitosan and ninhydrin. The linear range for determination of amino acid was 0.5 - 3.0 mmol/L,the limit of detection was 0.1 mmol/L. The total content of free amino acid in Hibiscus L.leaves was 10.18%, coefficient of variation was 1.3% and the recoveries of glutamic acid were in the range of 102.5 % - 94.3 %.

关 键 词：木槿叶 氨基酸 荧光猝灭法 壳聚糖 

分 类 号：O657.39[理学—分析化学]