B7反义肽预处理的受体树突状细胞抑制移植动脉的内膜增生  

Inhibition of the transplanted endarterium hyperplasia using the recipient dendritic cells pretreated by B7 antisense peptide

作　　者：姚宇锋[1] 项建斌[1] 蔡端[1] 

机构地区：[1]复旦大学附属华山医院外科,上海200040

出　　处：《复旦学报（医学版）》2007年第3期355-357,共3页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金(30400428);高等学校博士点科研基金(20030246069);复旦大学青年科学基金项目

摘　　要：目的建立同种异基因小鼠颈总动脉原位移植模型,探讨B7反义肽预处理的受体树突状细胞(DC)抑制移植动脉内膜增生的有效性。方法利用B7反义肽封闭负载供体抗原的受体DC,对受体C3H小鼠进行预处理后,建立异基因小鼠(Cm7BL／6→C3H)颈总动脉原位移植模型,2个月后对移植动脉作病理分析。结果DC预处理组移植动脉内膜增生减弱(P<0．05)．免疫组化分析示TGFβ1在动脉壁内的表达减少(P<0．05)。结论B7反义肽预处理的负载供体抗原的受体DC能够抑制移植动脉内膜的增生。Purpose A murine allograft model of carotid artery orthotopic transplantation was established to study the effect of the inhibition of the transplanted endarteriu.m hyperplasia using the recipient dendritic cells （DC） pretreated by B7 antisense peptide. Methods The reciepient was pretreated by B7 antisense peptide-blocked donor-pulsed recipient dentritic cells, then the allograft model of carot- id artery orthotopic transplantation was established（C57BL/6→C3H）. The transplanted artery was harvested to be analyzed pathologically after 2 months. Results The intima hyperplasia of the transplanted artery pretreated by the tolegenic recipient DC was weakened. And the expression of TGFβ1 also decreased according to immunohistochemistry analysis. Conclusions B7 antisense peptideblocked donor-pulsed recipient dentritic cells can inhibit the intima hyperplasia of the transplanted artery.

关 键 词：树突状细胞 慢性排异 动脉 移植 

分 类 号：R363[医药卫生—病理学] Q813.3[医药卫生—基础医学]