次氯酸钠对根管内粪肠球菌杀菌效果的体外实验  

In vitro evaluation of the antibacterial effectiveness of sodium hypochlorite on Enterococcus faecalis within root canals

作　　者：冯朝华[1] 高学军[2] 沈嵩[2] 

机构地区：[1]北京大学口腔医学院.口腔医院第一门诊部口腔内科,100034 [2]北京大学口腔医学院.口腔医院牙体牙髓科

出　　处：《中华口腔医学杂志》2007年第6期355-356,共2页Chinese Journal of Stomatology

基　　金："十五"国家科技攻关计划(2004BA720A23)

摘　　要：目的评价次氯酸钠对根管内粪肠球菌的杀菌效果。方法将45个离体前磨牙的感染根管标本分为6组,1、2组用5.25%及2.5%次氯酸钠冲洗,3组用0.9%NaCl 冲洗,4、5组在根管预备时辅以5.25%及2.5%次氯酸钠冲洗,6组在根管预备时辅以0.9%Nacl 冲洗。冲洗前、冲洗后即刻及冲洗后72h 分别取样培养。结果 6组根管内的细菌量均显著下降。1、2组差异无统计学意义(P>0.05),但均好于3组(P<0.05)。4、5和6组差异均有统计学意义(P<0.05)。根管冲洗后培养72h 均有细菌生长。结论 2.5%次氯酸钠基本可达到更高浓度的灭菌效果,但是经过机械预备和次氯酸钠化学消毒后的根管内仍有细菌残留。Objective To evaluate in vitro the antibacterial effectiveness of sodium hypochlorite on Enterococcusfaecalis（ E. faecalis ） within root canals. Methods Roots inoculated with E. faecalis were divided into six groups, which underwent different root canal irrigation and treatment： Group 1： 5. 25% NaOCl, Group 2： 2.5% NaOCl, Group 3： 0.9% NaCl, Group 4： root canal preparation ＋ 5.25% NaOCl, Group 5 ： root canal preparation ＋ 2. 5% NaOCl, Group 6 ： root canal preparation ＋ 0. 9% NaCl. Microbiological samples were collected from root canals at three time points （before irrigation, immediately after irrigation and three days after irrigation）. Results The numbers of E. faecalis in root canal in each of the six groups were effectively reduced. Group 1 and 2 were statistically more effective than Group 3. Group 4 was statistically more effective than Group 5 and 6. Group 5 was statistically more effective than Group 6. Bacterial survival was observed in all canals of each group after a 3-day-incubation. Condusions 2. 5% NaOCl was highly effective for root canal irrigation. However residual bacteria remained in the root canals after chemical irrigation by NaOCl and mechanical preparation.

关 键 词：次氯酸钠 根管冲洗剂 肠球菌 粪 

分 类 号：R781.05[医药卫生—口腔医学]