^99mTc标记抗β-人绒毛膜促性腺激素单克隆抗体  

Direct labeling of anti β-human chorionic gonadotropin monoclonal antibody with ^99mTc

作　　者：薛艳[1] 安瑞芳[1] 喻明启[2] 王姝[1] 谢丽[1] 

机构地区：[1]西安交通大学第一附属医院妇产科,710061 [2]西安交通大学第一附属医院核医学科,710061

出　　处：《国际放射医学核医学杂志》2007年第2期68-69,共2页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：陕西省科学技术研究发展计划基金资助项目（2003k10-G47）

摘　　要：目的用99mTc标记抗β-人绒毛膜促性腺激素（β-HCG）单克隆抗体。方法以SnCl2为还原剂,采用直接标记法进行标记。结果整个标记过程约需1.5h,抗体标记率为88.1%-97.5%,胶体含量为0.96%-1.27%,游离锝含量为1.10%-9.92%,且抗体的生物活性未受到影响。结论该法简单、快速,有望用于妊娠滋养细胞疾病的放射免疫显像。Objective To label the anti-β-human chorionic gonadotropin monoclonal antibody with β. Methods It was direct labeled using SnCl2 as a reduction agent. Results The labeling procedure was finished within 1.5 hours. The labeling efficiency of the labeled anti-β-human chofionic gonadotropin monoclonal antibody was 88.1%-97.5%, ^99mTc colloidal was 0.96%-1.27%, ^99mTcO4-was 1.1%-9.92%. And it retained its specific biological activation. Conclusion This direct method is simple, rapid and stable, and it hope to be used as radioimmunodiagnosis for gestational trophoblastic turnout.

关 键 词：锝 绒毛膜促性腺激素 Β亚单位 人 抗体 抗独特性 同位素标记 

分 类 号：R[医药卫生]