Tiam1基因过表达对结直肠癌细胞增殖和转移能力的影响  

Effect of Tiaml overexpression on proliferation and metastatic potential of human colorectal cancer

作　　者：刘莉[1] 许岸高[2] 张庆玲[1] 张艳飞[1] 丁彦青[1] 

机构地区：[1]南方医科大学南广医院病理科,广州510515 [2]广东省惠州市中心人民医院

出　　处：《中华病理学杂志》2007年第6期390-393,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金(30670967);国家自然科学基金青年基金资助项目(30500242);广东省自然科学基金(04006711)

摘　　要：目的探讨Tiam1(T lymphoma invasion and metastasis 1)基因转染对结直肠癌细胞增殖、转移能力的影响。方法采用裸鼠皮下接种结直肠癌细胞的方法观察Tiam1基因对结直肠癌细胞体内增殖能力的影响,利用外科原位移植技术观察Tiam1对体内增殖、转移能力的影响。结果接种HT29/Tiam1细胞(转染Tiam1基因)的裸鼠皮下肿瘤生长明显加快,肿瘤体积自皮下注射后的第7天起与接种HT29/mock细胞(转染对照)的裸鼠皮下肿瘤比差异有统计学意义,接种HT29/Tiam1细胞的裸鼠皮下肿瘤第20天的平均体积是接种HT29/mock细胞组的2.5倍。进行结直肠癌细胞外科原位移植6周后,接种HT29/Tiam1细胞的裸鼠结肠原位肿瘤重量明显犬于接种HT29/mock细胞的裸鼠(t=-14.916,P<0.01)。采用外科原位移植的方法比较HT29/mock和HT29/Tiam1细胞的体内转移能力。在HT29/Tiam1细胞组,7只裸鼠全都发生了腹腔转移,4只发生了肝转移,其中有1只裸鼠发生了广泛转移,包括脾、肺及淋巴结等转移。在HT29/mock细胞组,7只中只有2只裸鼠发生了腹腔转移,没有一只裸鼠发生远处转移。结论Tiam1基因是结直肠癌增殖、转移的促进基因,Tiam1表达可作为结直肠癌增殖、转移过程中一个有价值的指标。Objective To confirm the role of Tiaml （T lymphoma invasion and metastasis 1 ） gene in the proliferation and metastasis of colorectal cancer. Methods Proliferative and metastatic abilities of Tiaml transfectant were investigated by subcutaneous injection of cells and surgical orthotopic transplantation （SOI） in mice. Results The expression of Tiaml led to a pronounced increase in HT29/Tiaml cell growth starting from day 7, up to 2. 5 fold increase of tumor volume at day 20 post injection. Tumors in the HT29/ Tiaml group receiving surgical orthotopic implantation were significantly heavier than those in HT29/mock group （t = - 14. 916,P 〈0. 01 ）. In vivo metastasis assay by SOI showed that in HT29/Tiaml group, 7/7 of mice developed peritoneal metastases and 4/7 had hepatic lesions. In addition, one of the seven HT29/ Tiaml group mice had tumors in lung, spleen and lymph nodes. In the HT29/mock group, only 2/7 of animals had peritoneal metastases and none produced detectable tumor in the liver. Conclusions Tiaml gene plays an important role in the proliferation, invasion and metastasis of colorectal cancer. It may serve as a useful clinical marker for tumor progression and metastasis of colorectal cancer.

关 键 词：结直肠肿瘤 细胞增殖 肿瘤转移 细胞系 肿瘤 Tiaml 

分 类 号：R735.3[医药卫生—肿瘤]