三氧化二砷对CO_2气腹下小鼠肿瘤细胞CD44、VEGF表达影响的实验研究  

Experimental Study of Arsenic Trioxide on the Influence of Mouse Tumor Growth on Abdominal Wall and Trocar under Carbon Dioxide Pneumoperitoneum

作　　者：李鑫磊[1] 孙世波[2] 

机构地区：[1]大庆市人民医院普外科,黑龙江大庆163316 [2]哈尔滨医科大学附属二院普外科

出　　处：《中国伤残医学》2007年第3期9-10,共2页Chinese Journal of Trauma and Disability Medicine

摘　　要：目的:研究腹腔内注射三氧化二砷(As2O3)对小鼠CO2气腹下肝癌H22转移的影响。方法:昆明鼠40只(清洁级),中腹部穿刺置入1mm套管针,自套管针注入1×106肿瘤细胞后,建立CO2气腹,压力8mmHg,时间30min。术后随机分4组,每组10只,分别腹腔内注入:生理盐水,1ml;As2O3(2mg/kg),1ml;As2O3(4mg/kg),1ml;As2O3(4mg/kg)+肝素(10U/ml),共1ml。气腹后第3、7天测量CD44、VEGF的变化;比较各组生存状态、腹围、体重变化及转移瘤直径。结果:与对照组相比,As2O3组CD44、VEGF表达,转移瘤直径均明显降低。高剂量组比低剂量组效果稍好。As2O3肝素组戳口种植率最低。结论:As2O3对CO2气腹腹腔镜肿瘤生长转移有抑制作用。Objective： To study the influence of intraperitoneal instillation arsenic trioxide （AS2O3） on mouse hepatoma cell line H22 metastasis with mice under CO2 pneumoperitoneam. Methods： After l mm trocar was puncturaed through mid abdomen, l×10^6tumor cells were administered through l ram trocar on kun ming mice（n=40 clearing class）. Pneumoperitoneam was established over 30 min at 8 mm Hg with carbon dioxide. Mice were randomly divided into four groups （n=10） and received intraperitoneal instillation with one of the following： lmi of normal saline, l mi As203 （2 mg/kg）, l mi As203 （4 mg/kg）, or l ml As203 （4 mg/kg） with heparin （10 U/mi）. CD44, VEGF were tested in 3, 7 day after laparoscopy with CO2. It were also compared that the survival condition, the percentage of metastasis in trocar, variance of abdominal circumference and body weight. Results： Comparing with control group, there was lower expression of CD44 and VEGF, the percentage of metastasis in trocar, the metastatic tumor average diameter in As203 group. High-dose group has slightly better effective than the low-dose group. The lowest percentage of metastasis in trocar was founded in As203 with heparin group. Conclusions： As203 has the effect of inhibiting tumor growth and metabasis undergoing laparoscopy with CO2.

关 键 词：二氧化碳气腹 腹腔镜 黏附因子(cD44) 血管内皮生长因子(VEGF) 三氧化二砷(As203) 

分 类 号：R735.7[医药卫生—肿瘤]