共轭亚油酸对鹅抗氧化功能与脂质过氧化的影响  

Effects of Conjugated Linoleic Acid on the Anti-oxidation Function and Lipid Per-oxidation of Geese

作　　者：张旭晖[1] 王宝维[1] 王雷[1] 龙芳羽[1] 杨志刚[1] 刘光磊[2] 

机构地区：[1]莱阳农学院优质水禽研究所,青岛266109 [2]中国农业科学院畜牧研究所,北京100094

出　　处：《动物营养学报》2007年第3期299-304,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家"十五"科技攻关(编号:2004BA514A09-3);山东省农业良种产业化资助项目

摘　　要：本文旨在研究不同共轭亚油酸(CLA)添加水平对五龙鹅抗氧化性能与脂质过氧化的影响。选用1日龄五龙鹅192只,随机分为4个处理组,每个处理组48只,设4个重复,每个重复12只。在玉米-豆粕型日粮基础上分别添加CLA(0.0、5.0、15.0和25.0g/kg),以饲喂基础日粮组为对照组,试验期8周,测定并观察其在第2、4、6和8周龄血清中总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性、血清及肝脏组织中脂质过氧化物丙二醛(MDA)含量和总抗氧化能力(T-AOC)等各项指标的变化规律。结果表明:日粮添加5.0g/kgCLA时,能够显著提高鹅血清T-SOD活性(第6、8周龄)和血清(第6周龄)及肝脏组织中(第8周龄)T-AOC(P<0.05);添加15.0g/kgCLA,对鹅血清T-SOD,GSH-Px(第6、8周龄)活性及血清及肝脏T-AOC均有一定提高作用;而当添加水平为25.0g/kg时,对T-SOD,GSH-Px活性和T-AOC均有一定降低作用;不同添加水平CLA均能明显降低血清及肝脏中MDA含量(P<0.05)。本试验条件下,5.0g/kgCLA添加组对于提高和改善鹅的抗氧化功能与脂质过氧化状况效果最佳。To study the effects of different levels of dietary conjugated linoleic acid（CLA） on the anti-oxidation function and lipid per-oxidation of Wulong-geese,an experiment was conducted using a total of 192 one-day old Wulong-geese to investigate the effects of adding CLA oil （0.0,5. 0,15. 0,25.0 g/kg respectively） to corn soybean diets on the activities of T-SOD, GSH-Px in serum,and the contents of lipid per-oxidation of MDA and T-AOC in serum and liver at the end of 2 weeks （2w）, 4w,6w and 8w. Geese were randomly divided into 4 treatments （48 for each treatment, 12 for each replicate）. The basal diet was used as control group,and the experiment lasted for eight weeks. The result showed 1） the activities of T-SOD in serum （6w,8w） and T-AOC in serum （6w） and liver （8w） were increased significantly when the CLA additive level was 5.0 g/kg （P〈0.05）; 2） there were certain increase in the activities of serum T-SOD, GSH-Px （6w, 8w） and liver T-AOC when 15.0 g/kg CLA was added; 3） when 25.0 g/kg CLA was added,the activities of T-SOD,GSH-Px in serum and T-AOC all decreased to some extent; 4） the contents of MDA in serum and liver were reduced significantly in each CLA test group （P〈 0.05）. Under the condition of the experiment,it was better to add 5.0 g/kg CLA to the diets for the enhancement of antioxidation function and the improvement of capability of lipid per-oxidation status in geese.

关 键 词：CLA 抗氧化功能 脂质过氧化 鹅 

分 类 号：S858.31[农业科学—临床兽医学]