ZnCl_2与氧化胰岛素B链结合位点和切割位点的质谱指认(英文)  

Site-specific Hydrolytic Cleavage of Oxidized Insulin B Chain with Zn(Ⅱ) Ion Studied by Electrospray Ionization Mass Spectrometry and MS/MS Mass Spectrometry

作　　者：蒋疆[1] 梅毓华[2] 王文继[1] 吴琼洁[3] 朱龙根[3] 

机构地区：[1]福州大学化学与化工学院,福州350002 [2]南京大学配位化学国家重点实验室,南京210093 [3]福建农林大学生命科学学院,福州350002

出　　处：《无机化学学报》2007年第6期948-956,共9页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No.NSF20271027)。

摘　　要：采用电喷雾质谱和串联质谱研究了氧化胰岛素B链与ZnCl2的键合作用并成功地确定了切割位点。质谱研究显示在pH值2.5及40℃条件下,Zn2+通过与氧化胰岛素B链的氨基酸侧链His5,His10和Arg22结合,选择性地水解了肽键Asn3-Gln4,His5-Leu6,Gly8-Ser9和Glu21-Arg22。The bonding interaction and hydrolytic cleavage of oxidized insulin B chain with ZnC12 in molar ratios of 1：1 1：2 and 1：3 at pH value of 2.5 and 40℃ were investigated by electrospray ionization mass spectrometry and tandem mass spectrometry. The results show that the binding sites of Zn^2＋ with oxidized insulin B chain are Arg22 and imidazole groups of His5, His10, which leads to the selective cleavages of the peptide bonds at Asn3- Gln4, His5-Leu6, Gly8-Ser9 and Glu21-Arg22 of oxidized insulin B chain.

关 键 词：氧化胰岛素B链 质谱 ZNCL2 水解切割 

分 类 号：O657.6[理学—分析化学]