人卵巢癌SKOV3细胞多细胞团簇黏附性和转移性的体外研究  

The ability of adhesion and migration of SKOV3 spheroids in vitro

作　　者：刘召芬[1] 陈建利[1] 万小平[1] 

机构地区：[1]上海交通大学附属第一人民医院妇产科,上海200080

出　　处：《现代妇产科进展》2007年第5期341-344,I0001,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金(No:30371482);中国博士后科学基金资助课题(No:2005037141)

摘　　要：目的:探讨卵巢癌SKOV3多细胞团簇是否具有黏附性和转移性,以及单细胞和多细胞团簇黏附性和转移性的差异,以确定二者在卵巢癌扩散和转移中的作用。方法:用液体重叠培养系统获得SKOV3团簇,四甲基偶氮唑蓝(MTT)法测定SKOV3单细胞和团簇对细胞外基质(ECM)的黏附性;用Boyden小室和MTT法测定二者的转移性。结果:SKOV3团簇和单细胞均可黏附于ECM,黏附性对比中团簇组光吸收值(OD490)明显小于单细胞组(0.34±0.03vs0.57±0.77,P=0.000)。单细胞和团簇均可通过Boyden小室发生转移。团簇转移后的细胞以单细胞形式存在,光镜下观察转移后的两组细胞形态相似。团簇组转移细胞数明显少于单细胞组(35.00±30.00vs97.00±54.00,P=0.000);团簇组转移细胞的光吸收值(OD560)明显低于单细胞组(0.28±0.03vs0.37±0.10,P=0.005)。结论:卵巢癌多细胞团簇具有黏附性和转移性,其黏附性和转移性低于单细胞;以悬浮状态存在于卵巢癌患者腹水中的癌单细胞或多细胞团簇,在卵巢癌扩散和转移过程中均发挥着一定的作用。Objective： To study on the ability of adhesion and the ability of migration of SKOV3 spheroid and SKOV3 single cells in vitro, and determine whether both SKOV3 spheroids and single cells play a potential role in the process of ovarian cancer spread and metastasis. Methods：The spheroids were generated from the human ovarian carcinoma cell line SKOV3 by the liquid overlay technique. The abilitiy of adhesion of both spheroids and single cells was measured by MTT. The migration ability was determined by invasion chamber assay and MTT. Results：Both SKOV3 spheroids and single cells adhered to ECM. The OD490 of spheroids was much lower than that of single cells （ 0.34 ± 0.03 vs 0.57 ± 0.77, P = 0.000 ）. Both SKOV3 spheroids and single cells could migrate through a filter of Boyden chambers. The migrated cells in the spheroids group existed in single cell pattern and looked similar to that of migrated cells in the single cell group, The number of migrated cells of spheroids group （35.00 ± 30.00） was significantly lower than that of the single cells goup （97.00 ± 54.00 ） （ P = 0.000 ）. The OD560 of the migrated cells of spheroids group was much lower than that of single cells group （0.28 ± 0.03 vs 0.37 ± 0.10, P = 0.005 ）. Conclusions： Ovarian cancer multicellular spheroid have the ability of adhesion and the ability of migration. Both ovarian single cells and muhicellular spheroid should be regarded as potential contributors to disseminator of ovarian cancer.

关 键 词：卵巢肿瘤 多细胞团簇 黏附 迁移 细胞 

分 类 号：R737.31[医药卫生—肿瘤]