Nispex体内外抑制白血病细胞HL-60的生长  

Nispex inhibits myeloid leukemia cell HL-60 growth in vitro and in mice

作　　者：肖义军[1] 陈元仲[1] 陈炳华[2] 黄美娟[1] 俞萍丽[1] 林锦娟[1] 

机构地区：[1]福建医科大学附属协和医院福建省血液病研究所,福建福州350001 [2]福建师范大学生命科学学院细胞生物学实验室,福建福州350108

出　　处：《中国癌症杂志》2007年第6期461-465,共5页China Oncology

基　　金：福建省教育厅资助项目(项目号JA05201)

摘　　要：背景与目的:植物多酚类成分不仅有防癌抗癌作用,也可增强放化疗的效果,还可以保护正常细胞免受放化疗的毒性。本研究探讨一种主要成分为多酚的寄主为夹竹桃的红花桑寄生提取物(简称Nispex)的体内外抗急性髓性白血病HL-60的效果。方法:采用MTT法研究Nispex的体外抑瘤作用;以对小鼠的近似LD50值评估Nispex的急性毒性;以HL-60细胞裸鼠移植瘤模型为对象,研究Nispex及其与多柔比星(阿霉素)联用的体内抑瘤效果。以相对瘤体积和瘤重来评价药物的体内抑瘤效果,以药物相互作用指数(CDI)来评价药物联合作用疗效。结果:Nispex显著抑制HL-60细胞的增殖,存在明显的量效关系和时效关系,24、48、72和96h的IC50值分别是0.75、0.70、0.54和0.48μg/ml。Nispex对小鼠的近似LD50值为126.81mg/kg。Nispex(ip)-剂量为30mg/kg时,肿瘤生长抑制率达60.6%;10mg/kg与多柔比星联用时,肿瘤生长抑制率达94.4%,CDI=0.21<0.7,均有统计学意义。结论:Nispex体内外有一定的抑瘤效果,与多柔比星联用的协同作用非常显著,可能是一种有潜力的抗肿瘤植物提取物。Background and purpose： Many plant-derived polyphenols have been studied for their anticancer effects and potential chemopreventive properties. Recent researches have suggested that plant polyphenols might be used to sensitize tumor cells to chemotherapeutic agents and radiation therapy by inhibiting pathways that lead to treatment resistance. These plant polyphenols have also been found to protect normal cells from therapy-associated toxicities. Nispex is an extract of Scurrula parasitica L parasitizing on Nernium indicum Mill., consisting mainly of polyphenol such as quercetin, quercitroside, avicularin and oleandrin et al. The purpose of this study was to examine the anticancer activity of Nispex in vitro and in mice, and the synergistic anticancer effect of Nispex combined with adriamycin in mice. Methods： MTT assay was used to detect the growth inhibition of human acute myeloid leukemia cells HL-60 by Nispex treatment. Xenograft of HL-60 was established by subcutaneous implantation of cultured HL-60 cells in BALB/c nude mice. Tumor growth inhibition in these mice was used to evaluate the anticancer activity of drugs. Mice used in these experiments were randomly divided into six groups： control group, ADR group, 30 mg/（kg·day） Nispex（-ip） group, 10 mg/（kg·day） Nispex-ip group, 10 mg/ （kg · day） Nispex-it group, 10 mg/kg per day Nispex-ip combined with ADR group. ADR were administered through caudal vein once only on the first day, Nispex treatment, 14 days. Tumor growth inhibition was evaluated by relative tumor volume （RTV） and tumor weight （ TW）. Results： Nispex inhibited HL-60 cells proliferation significantly in time and dose dependent manner; IC50 values were 0.75, 0.70, 0.54 and 0.48 μg/ml after being treated at 24, 48, 72 and 96 hr by Nispex. HL-60 grafts of 30 mg/kg per day Nospex（ip） were inhibited by 60.6% according to RTV, 31.2% according to TW （P 〈0.05） ; grafts of 10mg/kg per day Nospex（it） were inhibited by 59.4% according to RTV, 45.3% according

关 键 词：红花桑寄生 夹竹桃 多酚 多柔比星 白血病 HL-60细胞 移植瘤 肿瘤联合化疗 

分 类 号：R733.7[医药卫生—肿瘤]