载5-FU免疫纳米微粒的体外释药及其抗癌效应研究  

Slow-Release In Vitro Characteristics and Anticancer Effects of 5-fluorouracil-Loaded Immunological Nanoparticles

作　　者：黄开红[1] 刘建化[1] 王凌云[1] 朱兆华[1] 陈其奎[1] 闵军[1] 陈汝福[1] 

机构地区：[1]中山大学附属第二医院消化内科,广州市510120

出　　处：《中国肿瘤临床》2007年第11期601-603,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金编号:30670951);广东省自然科学基金编号:06021322);广州市科技攻关项目基金资助编号:2003Z3-E0351

摘　　要：目的:研究生物可降解抗癌药载5-FU免疫纳米微粒的释药特点,鉴定其体外杀伤肿瘤细胞的活性。方法:采用恒温振荡透析法和一阶导数紫外分光光度法测定了载5-FU免疫纳米微粒的药物释放特性;MTT法测定了不同时相载5-FU免疫纳米微粒对5种肿瘤细胞株的杀伤活性。结果:载5-FU免疫纳米微粒具有良好的缓释功能,半量释放期t1/2为10.4天。载5-FU免疫纳米微粒对5种肿瘤细胞株均有较强的杀伤活性,且杀伤活性与作用时间和释药量呈正相关关系。结论:载5-FU免疫纳米微粒中药物分布均匀;载5-FU免疫纳米微粒制备和溶蚀过程对5Fu的药效无影响。载5-FU免疫纳米微粒具有良好的缓释功能。能在较长时间内维持有效的杀伤活性。Objective： To investigate the slow-release characteristics and antitumor effects of the bio-soluble 5-fluorouracil-loaded immunological nanoparticles, prepared by attaching anti-VEGF monoclonal antibodies to 5-fluorouracil-loaded polylactic acid nanoparticles （5-FU-PA-NPs）, in order to assess their anti-tumor activity in vitro. Methods： Constant temperature vibration dialysis and MTF colorimetric assays were used. Results： The 5-FU-PA-NPs showed appropriate extended release with a half-life of 10.4 days. There was a positive correlation between the anti-tumor activity and the reaction time and dose of the controlled release. Conclusion： The 5-FU-PA-NPs show a uniform drug deposition, and the therapeutic effect is not affected by preparation and metabolic breakdown of the drug. The 5-FU-PA-NPs have a well-controlled release which can maintain effective tumor-killing activity for an extended period.

关 键 词：载5-FU 免疫纳米微粒 缓释 抗癌效应 

分 类 号：R73-36[医药卫生—肿瘤]