两性霉素B缓释微球的制备及缓释性能研究  

Preparation and in vitro Release Behavior of Amphotericin B Loaded PLA-PEG Microspheres

作　　者：郁晓[1] 任杰[1] 任天斌[1] 袁华[1] 

机构地区：[1]上海同济大学材料学院纳米与生物高分子研究所,上海200092

出　　处：《中国生物医学工程学报》2007年第3期445-451,共7页Chinese Journal of Biomedical Engineering

基　　金：上海市科委纳米专项基金(0452nm059);同济大学工科基金。

摘　　要：为了更好地研究药物载体材料对药物微球缓释性能的影响,本研究将可完全生物降解的共聚物作为壁材以相分离法制备含抗真菌药物两性霉素B的微球,研究了不同溶剂/非溶剂、不同分子量共聚物、不同配比共聚物、不同表面活性剂及其不同用量等因素对微球的粒径大小、分布、药物包封率和药物体外释放等性能的影响。使用透射电镜(TEM)和原子力显微镜(AFM)观察微球的表面形貌,使用激光粒度分析仪测试微球的粒径大小及分布,使用紫外分光光度计测定药物的包封率。研究发现,聚合物特性粘度和分子量越大,聚合物中LA:PEG的配比越大,微球粒径越大,分布越宽;微球粒径越大,包封率也较大;AmB/PLA-PEG微球具有缓释性能,且含药微球的释放性能与微球的粒径,包封率等因素有关。Amphotericin B （AmB）-loaded poly （lactic acid）-poly （ethylene glycol） （PLA-PEG） microspheres were prepared by phase separation. The effect of the solvent/nonsolvent system, the composition of copolymer and the dosage of the Tween-80 on the size, the entrapment efficiency and the release behavior of the AmB loaded microspheres were investigated. The morphology of microspheres was characterized by atomic force microscopy （AFM） and transmission electron microscopy （TEM） respectively. The average particle size and size distribution were measured by laser light scattering technique. The entrapment ratio of AmB by microspheres was measured by UV spectrophotometer. It was found the microspheres size increased with the increase of LA/PEG weight ratio and the molecular weight of PEG segment in the PLA-PEG. The entrapment efficiency of AmB in PLA-PEG microspheres became higher and release rate became slower with the increase of LA/PEG ratio and addition of Tween-80.

关 键 词：聚乳酸-聚乙二醇共聚物 微球 相分离法 缓释 

分 类 号：R318.08[医药卫生—生物医学工程]