大鼠炎性神经根髓鞘形态学变化及复方中药栓剂对神经根损伤的修复  

Chinese herb suppository for the repair of nerve root injury and the morphological changes in the myeline sheath of rats with radicular neuritis

作　　者：罗才贵[1] 张冲[1] 罗建[1] 常德贵[2] 朱漓[1] 

机构地区：[1]成都中医药大学,四川省成都市610075 [2]成都中医药大学附属医院,四川省成都市610075

出　　处：《中国组织工程研究与临床康复》2007年第19期3748-3751,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：四川省科技厅资助课题(01SG003-06)~~

摘　　要：目的:观察腰痛灵栓直肠给药后神经根炎模型大鼠痛阈的恢复情况,髓鞘数量的变化以及神经根组织形态学改变与症状改善的相关性,探讨其作用机制。方法:实验于2004-04/05在成都中医药大学附属医院病理生理实验室完成。实验分组:取SD大鼠72只,雌雄各半,体质量150～180g,按性别体质量随机分为正常组、模型组、大剂量组、中剂量组、小剂量组、阳性对照组,每组12只。实验方法:建立大鼠化学神经根炎模型,模拟腰椎间盘突出症,腰痛灵栓直肠给药,大、中、小剂量组给腰痛灵栓分别为1.0,0.5,0.25g生药/kg,给药容量为0.35mL/kg;正常组、模型组给等容量的5g/L羧甲基纤维素钠溶液;阳性对照组给消炎痛栓5mg/kg,给药容量为0.35mL/kg,1次/d,连续给药20d。实验评估:各组分别在术后1,3,6,9,12,15,18d时观察记录大鼠的活动征象(痛阈)、髓鞘数量的变化,并检测再生神经的神经纤维髓鞘含量平均黑度、髓鞘积分吸光度、髓鞘总面积与神经组织截面积的百分比。结果:纳入SD大鼠72只,实验过程中模型组、小剂量组与阳性对照组有大鼠死亡,解剖发现手术处有脓肿形成3只(3组各1只),模型组不明原因死亡1只,为了提高统计效率,使各组动物数相等,在治疗结束检测前,随机剔除多余只数,最后每组10只,共60只大鼠进入结果分析。腰痛灵栓大剂量组大鼠的痛阈第9天恢复8只,第15天10只全部恢复,与阳性对照组比较差异有显著意义(P<0.05);腰痛灵栓大、中、小剂量组神经纤维髓鞘含量平均黑度分别为(98.33±24.84,83.42±26.34,75.10±13.69);髓鞘积分吸光度/截面积分别为(26.48±12.70,15.60±9.60,57.57±65.13);髓鞘总面积/截面积分别为(0.11±0.06,0.12±0.21,0.24±0.30),与模型组及正常组比较,差异均有显著意义(P<0.05)结论:①腰痛灵栓大剂量组能显著恢复模型大鼠的痛阈,并与剂量有关。②腰痛灵栓使大鼠神经根炎局部神经髓�AIM： To observe the recovery of pain threshold, alternation in quantity of myeline sheath, and association of histomorpholegical change in nerve roots with improvement of symptoms after rectal suppository （Yaotong/ing） that alleviates lumbago symptoms in rat model of radicular neuritis, and explore the action mechanism. METHODS： The experiment was carried out in the Pathophysiologic Laboratory of Affiliated Hospital of Chengdu University of Traditional Chinese Medicine from April to May 2004. Seventy-two SD rats of male and female in half and 150-180 g were selected and randomly divided into normal group, model group, high, moderate, and low doses groups, and positive control group with 12 in each group according to the body mass. After the rat models of chemical neuritis were established, 1.0, 0.5 and 0.35 mL/kg Yaotong/ing suppository （0.25 g crude drug/kg） was rectally injected in three doses groups respectively; the matching 5 g/L carboxymethylcellulose sodium liquid was injected to the normal control group and model group; the positive control group was treated wtih 0.35 mL/kg Xiaoyantong suppository （5 mg/kg）, once daily for concessive 20 days. The threshold of pain, and change in number of myeline sheath were observed on 1, 3, 6, 9, 12, 15, and 18 days after surgery; meanwhile, the average density of myeline sheath of regenerative nerves, absorbance of myeline sheath integral, and the ratio of total area of myeline sheath and sectional area of nerves were detected. RESULTS： Among the 72 rats, three of the model group, low dose group and positive control group died of infection or crud; one of the model group died of vague reasons. To raise the efficiency, the superfluous animals were randomly eliminated before the ending of treatment. Finally, 60 rats were involved in the result analysis with 10 in each group. The pain threshold of eight rats of the high dose group recovered on day 9, and all recovered on day 15, which was significantly different from the positive control group （P �

关 键 词：腰痛灵栓 神经根炎 髓鞘 

分 类 号：R745[医药卫生—神经病学与精神病学]