2,4,6-三硝基苯磺酸诱导小鼠炎症性肠病模型的建立  

Establishment of inflammatory bowel disease models induced by 2,4,6-trinitrobenzenesulfonic acid in mice

作　　者：陈佳奇[1] 陈村龙[1] 王继德[1] 唐建军[2] 姬宏莉[1] 

机构地区：[1]南方医科大学附属南方医院消化内科研究所,广东省广州市510515 [2]南方医科大学附属南方医院麻醉科,广东省广州市510515

出　　处：《中国组织工程研究与临床康复》2007年第21期4174-4177,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:建立2,4,6-三硝基苯磺酸诱导的结肠炎动物模型,并分析其剂量效应。方法:实验于2006-05/09在南方医院消化内科研究所完成,选用SPF级雌性Balb/c小鼠50只,按随机数字表法分为5组,每组10只,对照组予以300g/L乙醇溶液灌肠1次;其他4组分别给予含25mg/kg,50mg/kg,100mg/kg,150mg/kg2,4,6-三硝基苯磺酸的300g/L乙醇溶液灌肠1次。各组于造模后3,7,21,28d各处死1只小鼠,观察结肠的大体形态。造模后21d观察各组小鼠结肠的病理组织切片,并检测中性粒细胞的髓过氧化物酶活性。结果:造模过程中50,100,150mg/kg2,4,6-三硝基苯磺酸组各死亡2,3,3只小鼠。①各组小鼠造模后的一般情况:对照组及25mg/kg2,4,6-三硝基苯磺酸组小鼠发生一过性的结肠机械损伤表现,出现便血等现象,但3～7d后开始缓解;而50～150mg/kg2,4,6-三硝基苯磺酸组小鼠出现便血,腹泻。②各组小鼠结肠的大体形态及病理组织学观察结果:对照组和25mg/kg2,4,6-三硝基苯磺酸组小鼠肠道在第7天时表现为无粘连,局部充血,肠壁不增厚,未见溃疡,于第3周时局部充血已好转;结肠病理切片大致正常。50～150mg/kg2,4,6-三硝基苯磺酸组小鼠肠道肉眼可见粘连,肠腔增大,充血水肿,溃疡形成,出现肠道增厚,病理切片见黏膜下层水肿,固有层炎症细胞浸润等现象,以上现象于造模4周后开始缓解。其中当2,4,6-三硝基苯磺酸剂量为100mg/kg时作用达到高峰。③各组小鼠的中性粒细胞髓过氧化物酶活性比较:50,100,150mg/kg2,4,6-三硝基苯磺酸组均显著高于对照组[(14.17±3.33,18.50±2.33,18.17±1.50,4.83±2.00)nkat/L,(P<0.01)]。结论:应用含100mg/kg2,4,6-三硝基苯磺酸的300g/L乙醇溶液灌肠是诱导小鼠结肠炎较为理想的方法。AIM： To establish a 2, 4, 6-trinitrobenzenesulfnic acid （TNBS） induced colonitis model in mice and to analyze its dosage effect. METHODS： The experiment was performed at the Institute of Gastroenterology, Nanfang Hospital from May to September 2006. Totally 50 SPF-grade female Balb/c mice were selected and divided into 5 groups by random digits table, 10 mice in each group. The control group was instilled the 300 g/L ethanol once. The others groups were instilled by 25 mg/kg,50 mg/kg,100 mg/kgo150 mg/kg TNBS dissolved in 300 g/L ethanol into colon. One mouse was killed at days 3, 7, 21 and 28 after establishing models to observe the general formation of colon. 21 days after establishing models pathological tissue sections of colon were observed in each group. Myeloperoxidase （MPO） activity of neutrophilic leukocyte was evaluated. RESULTS： A total of 2, 3 and 3 mice were died in 50,100,150 mg/kg TNBS groups during establishment, respectively. （1）general condition： Once mechanical injury and hemafecia occurred in control group and 25 mg/kg TNBS group, but the symptom began to relieve 3-7 days later. Hemafecia and diarrhea were found in the 50-150 mg/kg TNBS groups. General form and observational findings of pathohistology： No adhesion, local congestion, no thickening of colon wall and no ulcer were seen in the control group arid 25 mg/kg TNBS group at day 7. The local congestion became improved and pathological sections were mostly normal at week 3. Adherence, incrassation, hyperemia, dropsy, ulceration, thickening of intestinal tract, and underlayer of mucosa was hydropsia, inflammatory cell infiltration in tunica propria, and above-mentioned phenomena became relieved from the 4^th week after establishing models in the 50-150 mg/kg TNBS groups. The effect reached the peak in 100 mg/kg TNBS group. （3）MPO activity in neutrophilic leukocyte： The activity was significantly increased in the 50, 100, 150 mg/kg TNBS groups as compared with the control group [（14.17±3.33, 18.50±2.33,18.17±

关 键 词：结肠炎 疾病模型 动物 过氧化物酶 

分 类 号：R574.62[医药卫生—消化系统]