软骨多糖对乳腺癌细胞CyclinD_1及p21周期蛋白表达影响的研究  

Impact of Cartilage Polysaccharide on Expression of CyclinD1 and p21 in Breast Cancer Cells

作　　者：贾永利[1] 张国蓉[1] 刘艳玲[1] 胡延勋[1] 刘安军[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《中国肿瘤》2007年第6期436-438,共3页China Cancer

基　　金：国家自然科学基金资助项目(20576101)

摘　　要：[目的]研究软骨多糖对乳腺癌细胞CyclinD1及p21周期蛋白表达的影响。[方法]选用MCF-7人乳腺癌细胞系进行体外培养,采用MTT法检测细胞增殖作用,流式细胞仪检测细胞周期的变化,免疫荧光染色方法检测细胞周期蛋白CyclinD1及p21蛋白的表达率。[结果]软骨多糖对MCF-7乳腺癌细胞体外生长有明显的抑制作用,且呈时间和剂量依赖性;软骨多糖使MCF-7乳腺癌细胞出现S期阻滞;软骨多糖促进CyclinD1蛋白失表达及p21蛋白过表达。[结论]软骨多糖通过影响MCF-7乳腺癌细胞周期蛋白的表达而诱导细胞凋亡,为抗乳腺癌活性物质。[Purpose ] To study the effect of cartilage polysaccharide on expression of CyclinD1 and p21 in breast cancer cells. [Methods] MCF-7 human breast cancer cells was cultured in vitro, cell proliferation effect was measured by MTT assay; cell cycle changes by flow eytometry; expression of CyclinD1 and p21 protein by immunofluorescent assay. [Results] Growth of MCF- 7 cells in vitro was significantly inhibited by cartilage polysaccharide with a time dependent and dose dependent manner. Cartilage polysaecharide induced cell cycle block in S phase and promoted low expression of CyelinDl and over expression of p21. [Conclusion] Cartilage polysaecharide plays anti-breast cancer effect with inducing apoptosis and changes of cell cycle in breast cancer cells.

关 键 词：乳腺肿瘤 细胞株 软骨多糖 CYCLIND1 :p21 

分 类 号：R73-354[医药卫生—肿瘤]