鹿狂犬病病毒G基因主要功能区的序列分析  

Sequence Analysis of the Main Regions in G Gene of Strain 8202 of Rabies Virus Isolated from Deer

作　　者：钱爱东[1] 侯世宽[1] 张茂林[1] 李红卫[1] 涂长春[1] 侯安祖 殷震[1] 

机构地区：[1]解放军农牧大学军事兽医研究所

出　　处：《中国兽医学报》1997年第3期209-214,共6页Chinese Journal of Veterinary Science

基　　金：军队医药卫生科研基金

摘　　要：通过反转录－聚合酶链反应（ＲＴ－ＰＣＲ）扩增出了中国鹿狂犬病野毒株（８２０２株）糖蛋白（Ｇ）基因膜外主要功能区的２个片段，共约８００个ｂｐ（３７０～１１７９），含有可以诱导中和抗体的２个抗原决定簇的基因片段和决定毒力及与神经细胞受体结合部位的基因片段。通过平端连接将２个片段克隆到ｐＵＣ１８的ＳｍａⅠ位点，采用Ｓａｎｇｅｒ双脱氧终止法测定ｃＤＮＡ片段的核苷酸序列，并推导出其氨基酸序列。将这一序列与国内外已发表的１１株狂犬病病毒的相应序列输入计算机进行比较分析，结果表明８２０２株主要功能区与上述１１个毒株核苷酸序列的同源性在７３．０％～９６．４％之间，氨基酸序列的同源性在８６．４％～９４．４％之间，８２０２株与中国疫苗株无论是核苷酸序列还是氨基酸序列的同源性均最高，而与中国街毒（ＣＤＸ－１）的核苷酸序列同源性最低，与ＣＶＳ株的氨基酸序列的同源性最低。Two fragments representing the main regions of glycoprotein (G) gene of deer rabies virus (strain 8202) were amplified by reverse transcriptase polymerase chain reaction(RT PCR) and cloned at Sma I site of pUC18 by blunt end ligation. The two fragments, which were responsible for the virulence of virus and the combination of virus with the receptor of nerve cell, as well as the induction of neutralization antibodies in host organism, are about 800 bp nucleotide(range from 370 to 1 179) in ectomembrane segment of G gene. The sequence of the cDNA was obtained by Sanger′s sequencing technique with their amino acid sequence being deduced. Comparison of the cDNA sequence and deduced amino acid sequence of strain 8202 with those of other 10 strains previously published in the world were performed by computer with DNASIS software. The results showed that the nucleotide homology of them ranged from 73.0% to 96.4% and the deduced amino acid sequence homology, from 86.4% to 94.4%. Both cDNA sequence and deduced amino acid sequence homology of strain 8202 with Chinese vaccine strain(strain 3aG) are the highest; while the nucleotide sequence homology of strain 8202 with Chinese street strain (CDX 1) is the lowest and the deduced amino acid sequence homology of strain 8202 with strain CVS is also the lowest. The results are important for the study on suitable genetic engineering vaccine against deer rabies and the investigation of molecular epidemiology of rabies virus.

关 键 词：鹿 狂犬病病毒 糖蛋白基因 序列分析 

分 类 号：S852.655[农业科学—基础兽医学]