流式细胞术检测GDNF在胶质瘤组织中的表达  

Expression of glial cell-derived neurotrophic factor in human brain gliomas and its significance

作　　者：司志超[1] 王冰[2] 庞琦[1] 葛明旭[1] 翟国德[1] 王汉斌[1] 孔大伟[1] 张康[1] 

机构地区：[1]山东大学山东省立医院神经外科,山东济南250021 [2]山东大学山东省立医院眼科,山东济南250021

出　　处：《中华肿瘤防治杂志》2007年第10期752-754,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的：探讨胶质细胞源性神经营养因子（glial cell-derived neurotrophic factor,GDNF）表达与人脑胶质细胞瘤恶性程度的关系。方法：应用流式细胞术的方法检测GDNF在人脑胶质瘤组织中的表达水平,进行免疫荧光显微镜观察和单参数分析。结果：GDNF在人脑胶质瘤和正常脑组织中均有表达,其中阳性表达率在胶质瘤组织中分别为41.17%、41.67%、68.75%和100.00%,正常脑组织为45.00%,计算所得FI值分别为0.83-1.07、0.82-1.10、0.96-1.78和1.47-2.16,正常脑组织为0.58-0.89,显示细胞荧光强度逐渐增加。对所得数据行方差分析,各样本采用方差齐性检验,显示各样本均数差异有统计学意义,P=0.0678。样本均数间采用LSD-t检验进行两两比较显示GDNF在胶质瘤中的的表达水平显著高于正常脑组织,且随着胶质瘤恶性程度的增加表达水平也增加（P〈0.01）。结论：GDNF可能作为一种重要的因素参与胶质瘤的发生、发展及分化,其可以作为胶质瘤的检测指标,提示胶质瘤的病理分级。为进一步研究GDNF的作用及作用机制,以及探讨人脑胶质瘤的发生、发展提供了新的有意义的理论依据。OBJECTIVE： To study the relationship between the expression of GDNF and malignancy of human brain gliomas. METHODS： By using flow cytometry, the expressing level of GDNF in human brain gliomas was detected. The data obtained under a fluorescence microscope were processed by the one-parameter analysis. RESULTS： The level in gliomas specimens was much higher than that in NBT. The different expressions were detected in different-malignancy gliomas too： the higher malignancy, the higher level. The differentiations of positive rates （41.17%, 41.67%, 68.75%, 100.00% and 45.00%） and FIdata （0.83-1.07, 0.82-1. 10, 0.96-1.78, 1.47-2.16 and 0. 58-0. 89） were evident. For all gliomas and NBT the homogeneity test of variance and ANOVA was calculated,P=0. 067 8. And the variance was considered significant. The pairwise comparison was analyzed by LSD-t test between the sample means（P〈0.01）. CONCLUSIONS： GDNF may participate in the occurrence, development and differentiation of glioma as an important factor. The results provide a reliable standard to distinguish the malignancy of gliomas or pathology classification. The results also provide a new significant theory evidence for studying the action and action mechanism, exploring the origination and development of human brain gliomas.

关 键 词：神经胶质瘤 脑源性神经营养因子 流式细胞术 

分 类 号：R739.4[医药卫生—肿瘤]