骨肉瘤组织中VEGF和BMP-2表达及微血管密度与转移关系的研究  

Experssions of vascular endothelial growth factor and bone morphogenetic protein-2 and microvessel density in osteosar-comas and their correlation with metastasis

作　　者：刘吉兵[1] 李敏[1] 原晓红[2] 高彦[3] 

机构地区：[1]山东省肿瘤医院骨软科,山东济南250117 [2]莱州市人民医院外科,山东莱州261400 [3]山东省肿瘤医院病理科,山东济南250117

出　　处：《中华肿瘤防治杂志》2007年第10期776-778,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究骨肉瘤组织中VEGF和BMP-2的表达及微血管密度(microvessel density,MVD)与转移的关系。方法:用免疫组织化学法检测28例骨肉瘤组织中VEGF、BMP-2的表达和MVD。结果:骨肉瘤组织中VEGF的阳性信号主要位于细胞胞质,BMP阳性信号呈胞质型。VEGF、BMP-2表达和MVD与肿瘤转移密切相关(χ2=15.731,P=0.000;χ2=9.257,P=0.006;t=6.071,P=0.001),VEGF表达与MVD密切有关(t=3.677,P=0.006),VEGF的表达强度与BMP-2表达密切有关(χ2=9.257,P=0.006)。结论:在骨肉瘤的发生发展中VEGF是促进微血管生成的主要细胞因子;VEGF可能影响BMP的骨诱导过程;VEGF和BMP的表达水平与骨肉瘤的转移发生有关,检测VEGF表达BMP-2与MVD值可作为判断骨肿瘤预后的重要指标。OBJECTIVE：To study the expressions of vascular endothelial growth factor and bone morphogenetic protein and microvessel density in osteosarcomas and their correlation with metastasis. METHODS： The immunohistochemistry technique was used to detect the expressions of VEGF and bone morphogenetic protein-2 and MV density in 28 osteosarcoma tissues. RESULTS： In osteosarcoma tissues, the positive signal of VEGF was mainly localized in cytoplasma of tumor cells. The positive signal of bone morphogenetic protein-2 was localized in cytoplasma. VEGF expression, BMP-2 expression and MVD had significant correlation with metastasis Х^2=15. 731, P= 0.000：Х^2=9.257,P=0.006; t=6.071,P=0.001）. VEGF expression had significant correlation with MVD （t = 3. 677, P = 0. 006）. VEGF expression had significant correlation with BMP-2 expression （Х^2= 9. 257, P = 0. 006）. CONLUSlONS： VEGF may be a main factor stimulating angiogenesis in osteosarcoma genesis and development. It is possible that VEGF has an influence on the bone-forming progression of BMP. The expression levels of VEGF and bone morphogenetic protein-2 were related to metastasis of osteosarcoma. Detection of VEGF and BMP-2 expressions and MV density may be useful for judging prognosis of malignant bone tumors.

关 键 词：骨肉瘤 血管内皮生长因子 BMP-2 微血管密度 免疫组织化学 转移 

分 类 号：R738[医药卫生—肿瘤]