人肺癌X和Y染色体异倍性的研究  

Study on the chromosome aneuploid of X and Y in human lung cancer

作　　者：高树庚[1] 董向阳[1] 汪良骏[1] 赫捷[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院胸外科,北京100021

出　　处：《中华肿瘤防治杂志》2007年第12期928-929,共2页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨间期核荧光原位杂交(FISH)检测肺癌印片标本X和Y染色体异倍性的可行性。方法:选取X和Y染色体特异着丝粒探针,用FISH技术检测46例肺癌印片标本中X和Y染色体异倍性。结果:80·43%(37/46)的标本中发现X和(或)Y染色体异倍体,主要特征为X染色体增多和Y染色体丢失。X染色体增多和Y染色体减少的发生比例分别为:71·74%(33/46)和54·05%(20/37)。结论:间期核FISH可以检测肺癌印片标本中X和Y染色体异倍体,应对X和Y染色体异倍性的临床意义进一步研究。OBJECTIVE： To study the feasibility of interphase fluorescence in situ hybridization （FISH） in detection of chromosomal aneuploidy of X and Y in touch preparations of lung cancer. METHODS： Touch preparations of 46 cases with lung cancer were examined by FISH with chromosomes X and Y specific centromere probes. RESULTS： Aeulpoidy, mainly featured in gains of chromosomal X and loss of chromosome Y were observed in 80. 43% of 46 cases. Gains of X and loss of Y were 71.74% （33/46） and 54. 05%（20/37）, respectively. CONCLUSIONS： Interphase FISH is feasible in detecting aneuploidy of chromosome X and Y, we shuld perform further study on clinic significance of X and Y aneuploidy.

关 键 词：肺肿瘤 原位杂交 荧光 染色体 

分 类 号：R734.2[医药卫生—肿瘤]