C57BL6近交系小鼠前列腺癌原位移植瘤模型的建立  

Establishment of the prostate cancer orthotopic implantation model in C57BL6 syngenic mice and its value of application

作　　者：郭宝锋[1] 张灵[2] 赵丽娟[2] 汲坤[2] 王金国[2] 赵雪俭[2] 

机构地区：[1]吉林大学中日联谊医院,长春130033 [2]吉林大学前列腺疾病防治研究中心

出　　处：《中国男科学杂志》2007年第5期5-8,共4页Chinese Journal of Andrology

基　　金：本课题受中日政府间专项技术合作项目基金资助(第59项)科技部国际科技合作重点项目(2004DFB02000)

摘　　要：目的建立C57BL6近交系小鼠前列腺癌原位移植瘤模型,观察在体前列腺癌生长转移的生物学特性。方法利用显微外科技术,将保持了完整组织结构的、移植于C57BL6近交系小鼠皮下的小鼠前列腺癌块,植于C57BL6近交系小鼠前列腺两腹侧叶之间,观察移植后肿瘤转移情况及小鼠的生存期。结果C57BL6进行RM-1肿瘤块原位移植术后,第15～20天,动物出现一般状态衰竭,体重下降明显,下腹可触及包块,全部为肿瘤占据,前列腺组织见不到。原位模型组大体见癌组织广泛地浸润周围盆腔脏器,其中淋巴结(16/16)、肺部(8/16)转移。两种模型荷瘤鼠生存时间原位模型组小鼠(17.0±3.5)d短于皮下模型组(33.0±5.6)d,两者相比差异有显著性。结论本模型可较好地模拟小鼠前列腺癌体内的自然生长状况,适合用于观察前列腺癌在体条件下的生物学特性。Objective To establish the prostate cancer orthotopic （o. t.） implantation model in C57BL6 syngenic mice and discuss its value of application. Methods The o. t. implantation model was established by subcapsular implantation of histologically intact tumor tissues harvested from a subcutaneous tumor by RM-1 cells into the protate gland of C57BL6 syngenic mice using microsyringe. The subcutaneous （s. b.） implantation model was used as control. The rate of metastasis and the survival time of these two models were compared. Results Metastasis occurred in all mice of the o. t. model with the highest rate of pelvic lymph node （16/16） and the lung （8/16）. No metastasis was seen in the control group except local growth and infiltration. The survival time of o. t. model was （17.0±3.5） days, markedly shorter than that of s. b. model （33.0 ±5.6） days. Conclusion The prostate cancer o. t. implantation model is an optimal clinical animal model to observe metastasis that mimics the natural situation of prostate cancer in human body quite well.

关 键 词：前列腺肿瘤 肿瘤移植 肿瘤转移 模型 动物 

分 类 号：R737.25[医药卫生—肿瘤]