红藻氨酸致癎大鼠神经元凋亡的动态研究  

A study of neuronal apoptosis after kainic acid-evoked seizures in rats

作　　者：吴原[1] 庞家容[1] 郑金瓯[1] 邓晓清[1] 梁秀琳[1] 李佳荃[2] 陈志颖[3] 

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁530022 [2]广西医科大学科学实验中心 [3]广西医科大学第一附属医院脑电图室,广西南宁530022

出　　处：《临床神经电生理学杂志》2007年第3期131-134,共4页Journal of Clinical Electroneurophysiology

摘　　要：目的:探讨红藻氨酸(kainic acid,KA)诱导的癫癎大鼠脑内神经元凋亡在不同部位和致癎后不同时间的变化情况。方法:将50只SD大鼠随机分为对照组和KA组,KA组再按癫癎发作后1天、1周、2周、3周和4周不同时点分为5个亚组。KA注射后,观察大鼠癫癎发作后的行为学变化,采用原位细胞凋亡检测法观察不同部位、不同时点神经元凋亡情况。结果:KA注射后,大鼠出现严重的惊厥,在癫癎发作后1天、1周和4周凋亡细胞明显增多,以齿状回、丘脑和CA3区明显(P<0.05)。结论:细胞凋亡贯穿KA致癎大鼠癫癎发作后神经元迟发性死亡的全过程。Objective：To study the spatio-temporal profile of brain neuronal apoptosis after epileptic seizures in rats. Methods： Sixty-five SD rats were classified as the control group and the kainic acid（KA） group. The KA group was also divided into 5 sub-groups at different time （1d,1w,2w,3w and 4w） after epileptic seizures. The change of ethology was observed after epileptic seizure was induced by KA injection,and the change of neuronal apoptosis at different time and in different parts through terminal deoxynucleotidy 1 transferase Dutp nick-end labeling （TUNEL stain） . Results： Severe seizures appeared in rats after K A injection, and there were more sporadic positive cells in l d , lw and 4w after seizures . The numbers of positive cells of dentate band , cerebral ganglion and CA3 sectors significantly increased（P〈 0.05）. Conclusions： Apoptosis participates in the process of delayed neuronal death of rats in KA-evoked seizures.

关 键 词：癫痫 红藻氨酸 细胞凋亡 

分 类 号：R742.1[医药卫生—神经病学与精神病学]