不同辐射抗拒鼻咽癌细胞微小RNA差异表达的研究  

Discrepancy of microRNA in different radioresistant nasopharyngeal carcinoma cells

作　　者：王旭丹[1] 杨惠玲[1] 郭禹标[2] 梁志慧[3] 赵睿颖 夏云飞[3] 苏勇[3] Mong-Hong Lee 王瑾[1] 郑芹[1] 

机构地区：[1]中山大学中山医学院病理生理学教研室 [2]中山大学附属一院内科 [3]中山大学肿瘤防治中心 [4]Department of Molecular and Cellular Oncology,University of Texas,MD Anderson Cancer Center,Houston TX 77030,USA

出　　处：《中国病理生理杂志》2007年第6期1045-1048,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30670627);广东自然科学基金资助项目(No.06021210);广东重大科技专项基金资助项目(No.30604);广东省重点科技攻关资助项目(No.2004A30801006);广州市科技计划(国际合作)资助项目(No.2004J1-I0041)

摘　　要：目的:在验证鼻咽癌(nasopharygycarcinoma,NPC)细胞CNE-1和CNE-2不同辐射抗拒性的基础上,探索微小RNA(miRNA)在CNE-1和CNE-2中的表达差异。方法:通过观察X线照射对CNE-1和CNE-2细胞克隆形成数目的影响,利用SigmaPlot软件进行分析及线性二次模型拟合存活曲线,比较CNE-1和CNE-2细胞剂量存活曲线及其生物学参数;采用Paraflo microfluidic microRNA芯片检测,用激光扫描器收集杂交的图像,经LOWESS滤器规范信号后分析数据差异,根据Targetscan3·1数据库资料(http:∥www·targetscan·org),预测CNE-1和CNE-2细胞microRNA差异表达与NPC放射敏感性差异的关系。结果:发现在CNE-1和CNE-2细胞中miRNA的差异表达,即与CNE-2细胞比,在检测的326个microRNA中,CNE-1细胞中有20个miRNA上调,13个miRNA下调,其中检测量的绝对值在2000以上且两者相差在3倍以上的miRNA有hsa-miR-152、hsa-miR-7、hsa-miR-205和hsa-miR-572;分析结果提示明显差异表达的miRNA与放疗敏感性密切相关。结论:不同辐射抗拒NPC细胞株CNE-1和CNE-2细胞的microRNA的表达有差异;差异表达的miRNA与放疗敏感有关。AIM： To discuss the discrepancy of microRNA （ miRNA ） in nasopharyngeal carcinoma （ NPC ） cells CNE - 1 and CNE - 2 on the basis of validating their different radioresistance. METHODS ： Following the effect of X ray on the clones of CNE - 1 and CNE - 2 cells, the dose - survival curve and biological characteristics of CNE - 1 and CNE -2 cells were determined by SigmaPlot software and the linear quadratic model of survival curves analysis. MicroRNAs were detected by Paraflo microfluidic microRNA chip, hybridization images collected using a laser scanner and normalizing the signals using a LOWESS filter. The relationship between the discrepancy of NPC radiosensitivity and the expression of microRNA was predicted according to Targetscan3.1 database （ http：//www, targetscan, org） after analyzing the data. RESULTS： Compared to CNE -2 cells, 20 microRNAs were gain -of- function and 13 microRNAs loss -of- function in CNE - 1 cells among 326 detected microRNAs. 4 miRNAs that one detective value was more than 2000 and 3 folds than the other were hsa - miR - 152, hsa - miR - 7, hsa - miR - 205 and hsa - miR - 572. Data showed that radio - sensitivity was relative to the distinct discrepancy of miRNAs. CONCLUSION： The discrepancy of miRNAs is presents in different radioresistant NPC cell lines and related to radiosensitivity.

关 键 词：鼻咽肿瘤 CNE-1细胞 CNE-2细胞 MICRORNA 辐射 

分 类 号：R363[医药卫生—病理学]