钙敏感受体在大鼠心肌缺血/再灌注损伤的表达变化及与心肌损伤关系  

Expression of calcium sensing receptor during ischemia/reperfusion myocardial damage and relationship between CaSR and injury of myocardium

作　　者：姜春明[1] 韩丽萍[1] 李鸿珠[1] 徐长庆[1] 孙轶华[1] 赵炜明[2] 

机构地区：[1]哈尔滨医科大学病理生理学教研室 [2]哈尔滨医科大学生物化学教研室

出　　处：《中国病理生理杂志》2007年第6期1084-1087,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370577;No.30470688);黑龙江省研究生创新科研资金资助项目

摘　　要：目的:观察钙敏感受体(CaSR)在大鼠心肌缺血/再灌注时的表达变化,揭示其与心肌缺血/再灌注损伤的关系。方法:Wistar大鼠随机分为5组:假手术组(sham组)、缺血再灌注1、2、4和6h组(I/R1h、2h、4h、6h组)。采用冠状动脉结扎和松结的方法,复制大鼠在体心肌缺血/再灌注损伤模型,记录左室收缩压(LVSP)和左室内压最大变化速率(±dp/dtmax),测定血清LDH、SOD活性和MDA含量,透射电镜观察心肌超微结构变化,RT-PCR法检测心肌组织中CaSR mRNA的表达变化。结果:LVSP、左室内压±dp/dtmax及SOD活性随再灌注时间延长而减低,LDH活性和MDA含量在再灌注2h时最高;心肌超微结构损伤在再灌注1h、2h较重,随再灌注时间延长而减轻;大鼠心肌缺血/再灌注1h、2h心肌组织CaSR的mRNA表达升高,再灌注4h、6h后降低。结论:CaSR mRNA表达多时心肌损伤较重,CaSR可能参与了心肌缺血/再灌注损伤。AIM： To investigate the expression of calcium sensing receptor（CaSR） during myocardial injury induced by ischemia/repedusion and disclose the relationship between CaSR and myocardial ischemia/reperfusion. METHODS： The experimental model was established by the 30 rain ligating and 1 h, 2 h, 4 h, 6 h reperfusing the left descending coronary artery （LAD） in rats. Wistar rats were randomly divided into 5 groups： sham group, ischemia/reper- fusion 1 h, 2 h, 4 h, 6 h groups （I/R 1 h, 2 h, 4 h, 6 h group）. CaSR mRNA expression was detected by RT-PCR. Left ventricular function was recorded. The levels of plasma lactate dehydrogenase （ LDH）, alondialdehyde （MDA） and superoxide dismutase （SOD） were measured. The change of ultrastructure in the ischemia/reperfusion myocardium of rats was observed by electron microscopy. RESULTS： LVSP, ± dp/dtmax and SOD activity decreased gradually with the reperfusion time prolonged. LDH and MDA peaked at 2 h. The ultramicro - structural injury at the 1 h and 2 h was more serious than that at 4 h and 6 h. The expression of CaSR increased significantly after reperfusion of 1 h and 2 h, and decreased after 4 h and 6 h. CONCLUSION： The increased expression of CaSR mRNA and serious injure of myocardium were observed. CaSR may be associated with the myocardial ischemia/reperfusion injury.

关 键 词：心肌再灌注损伤 受体 钙敏感 自由基 

分 类 号：R363[医药卫生—病理学]