厄贝沙坦对早期糖尿病肾病大鼠足细胞nephrin表达的影响  

Effect of irbesartan on nephrin expression in the podocyte of early diabetic nephropathy rats

作　　者：龙海波[1] 钟娟[1] 魏连波[1] 周伟东[1] 许兆忠[2] 

机构地区：[1]南方医科大学珠江医院中西医结合肾病中心,广东广州510282 [2]南方医科大学珠江医院急诊科,广东广州510282

出　　处：《中国病理生理杂志》2007年第6期1176-1180,共5页Chinese Journal of Pathophysiology

基　　金：南方医科大学珠江医院院长基金资助项目(No.200403)

摘　　要：目的:观察nephrin在糖尿病肾病(DN)大鼠肾小球足细胞中的表达,探讨厄贝沙坦对DN肾脏保护作用的机制。方法:应用链脲佐菌素建立大鼠DN模型,将DN模型大鼠随机分为2组:厄贝沙坦治疗组、模型对照组;另设正常对照组。各组分别干预8周后,观察大鼠体重、肾重、相对肾重、24h尿蛋白定量、血糖、血清尿素氮、肌酐、总胆固醇、甘油三酯变化,利用光镜、电镜观察肾脏病理改变,应用免疫组化技术观察nephrin表达情况。结果:应用厄贝沙坦干预后,DN大鼠24h尿蛋白定量明显减少、肾功能改善、肾脏病理改变显著减轻、足细胞nephrin表达量明显减少。结论:厄贝沙坦对DN肾脏的保护作用可能与其抑制足细胞nephrin表达有关。AIM： To investigate the expression of the nephrin in pedocyte of the diabetic nephropathy（DN） rats and the mechanism of irbesartan - induced renal protection. METHODS ： The DN model was established by a single injection of streptozotocin（STZ）,and DN rats were randomly divided into 2 groups： model group and irbesartan treatment group. In addition,the normal rats served as a normal control group. All the rats were received daily gavage respectively for 8 weeks. The urinary protein quality in 24 hours, body weight（BW） , kidney weight （KW）, KW/BW, glucemia ,urea nitro- gen, creatinine, total cholesterol, triacylglycerol were detected with correlative methods and the pathological changes of kidney were also detected with optic microscope and transmission eleftron microscope. The expression of nephrin in pedocyte were detected by immunohistochemistry. RESULTS ： In DN rots, irbesartan reduced the urinary protein quality in 24 hours （ P 〈 0.01 ） and alleviated the damage of kidney. Meanwhile, the expression of nephrin was declined remarkably in podocytes in irbesartan treatment group compared with model group（ P 〈 0. 05 ）. CONCLUSION： Irbesartan might protect kidney against STZ - induced injury via decreasing the expression of nephrin in podocytes.

关 键 词：糖尿病肾病 血管紧张素类 厄贝沙坦 足细胞 NEPHRIN 

分 类 号：R363[医药卫生—病理学]