多西环素诱导单核细胞系THP-1凋亡的实验研究  

Doxycycline induces apoptosis in THP-1 cells

作　　者：邹文进[1] 刘祖国[1] 蒋爱华[1] 李朝阳[1] 

机构地区：[1]中山大学中山眼科中心眼科学国家重点实验室,广东广州510060

出　　处：《中国病理生理杂志》2007年第6期1195-1198,共4页Chinese Journal of Pathophysiology

基　　金：国家"十五"科技攻关计划资助项目(No.2004BA720A15);国家自然科学基金群体创新项目资助(No.303210004);国家自然科学基金资助项目(No.30572001)

摘　　要：目的:研究多西环素诱导THP-1细胞凋亡的作用。方法:以PMA诱导THP-1细胞分化贴壁,分别按0mg/L、5mg/L、10mg/L、20mg/L、40mg/L、80mg/L的浓度梯度加入多西环素,作用12h或48h,设空白对照组。观察细胞形态变化,采用MTT法检测THP-1细胞活性,AnnexinⅤ和PI双染色后用流式细胞仪检测THP-1细胞早期凋亡,末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)检测THP-1细胞晚期凋亡。结果:随着多西环素浓度增高,THP-1细胞收缩变圆,细胞间距增宽乃至完全分离,死亡、崩解的THP-1细胞逐渐增多。MTT法显示随着多西环素作用浓度增高,活性THP-1细胞数量逐渐下降。流式细胞仪检测和TUNEL检测显示THP-1细胞的早期凋亡和晚期凋亡率均伴随多西环素作用浓度的升高而升高。结论:本实验首次阐明多西环素可以诱导人THP-1细胞凋亡,其效应呈明显的剂量依赖关系。AIM： This study was designed to investigate the apoptotic effect of doxycycline in THP - 1 cells. METHODS： After differentiated by PMA, THP - 1 cells were treated with doxycycline at different concentrations ranging from zero to 80 mg/L. The morphological changes of THP - 1 cells were observed under light microscope. MTr assay were used to examine the effects of doxycycline on proliferation of THP - 1 cells. Apoptotic THP - 1 cells were measured by An- nexin - V flow cytometry analysis and TdT - mediated dUTP nick end labeling assay. RESULTS： Treated with a certain concentration of doxycycline, differentiated THP - 1 cells contracted and turn round, a number of cells were dead. MTT assay and positive Annexin V - FITC on cell membrane and TUNEL assay showed that doxycycline induced apoptesis in THP - 1 cells in a dose - dependent manner. CONCLUSION： Doxycycline induces apoptosis in THP - 1 cells in a dose - dependent manner.

关 键 词：多西环素 单核细胞 细胞凋亡 

分 类 号：R363[医药卫生—病理学]