Ⅱ相代谢酶基因多态性联合烟酒习惯对肝癌易感性的影响  

Effect of interaction between smoking,alcohol drinking and polymorphisms of phase Ⅱ metabolic genes on the susceptibility of hepatocellular carcinoma

作　　者：贺菽嘉[1] 顾永耀[2] 廖志红[1] 

机构地区：[1]广西医科大学基础医学院生物化学与分子生物学教研室,广西南宁530021 [2]广西医科大学基础医学院病理学教研室,广西南宁530021

出　　处：《基础医学与临床》2007年第6期666-669,共4页Basic and Clinical Medicine

摘　　要：目的探讨Ⅱ相代谢酶（GSTM1、EPHX1）基因多态性和烟酒习惯及其相互作用与肝癌的关系。方法采用多重PCR、PCR-RFLP对广西地区105例肝癌患者及151例健康对照的GSTM1、EPHX1基因型进行检测，并调查研究对象的烟酒习惯。结果GSTM1缺失基因型频率在病例组与对照组中分别为64．76％和50．99％（P〈0．05）；EPHX1Tyr／Tyr、Tyr／His、His／His三种基因型频率在病例组分别为27．62％、21．90％和50．48％，对照组则分别为21．19％、34．44％和44．37％，两组间无差异。GSTM1缺失基因型与吸烟、饮酒因素在肝癌的发生中有交互作用，OR分别为3．13（95％CI：1．48—6．61）和4．27（95％CI：1．82—10．02）；吸烟者EPHX1基因型为His／His的个体发生肝癌的危险性增加，其OR为2．99（95％CI：1．29—6．91）。结论GSTM1是肝癌的遗传易感因素，Ⅱ相代谢酶基因多态和烟酒习惯的联合在肝癌的发生中起交互作用。Objective This study intended to explore the relationship of the polymorphisms of phase Ⅱ metabolic genes （ GSTMI and EPHXI ）, smoking, alcohol drinking and their interactions on risk of hepatocellular carcinoma （HCC）. Methods Using multiplex PCR and PCR-RFLP, the genotypes of GSTMI and EPHXI were analyzed in 105 patients with HCC and 151 health controls in Guangxi. The state of smoking and alcohol drinking were investigated. Results The frequency of the GSTM1 null genotype in cases was 64. 76% and 50. 99% in controls, which was significantly different （P 〈 0. 05, OR = 1.77 ）. The frequencies of 3 genotypes for EPHX1 in cases were 27. 62% ,21.90% and 50.48%, while those of controls were 21.19% ,34. 44% and 44. 37%. The difference was not statistically significant. Both smoking and alcohol drinking were respectively interacted with GSTM1 null genotype, with the odds ratios of 3. 13（95% CI：1. 48 -6. 61 ） and 4. 27（95% CI：1. 82 ~ 10. 02）. The combination of smoking and His/His of EPHXI increased the risk of HCC, which OR was 2. 99（95% CI：1. 29 -6. 91 ）. Conclusion GSTM1 is a susceptible genetic factor to HCC, smoking, alcohol drinking and polymorphisms of phase Ⅱ metabolic genes could play a cooperative effect to HCC.

关 键 词：肝癌 谷胱甘肽硫转移酶M1 微粒体环氧化物水解酶 多态性 烟酒习惯 

分 类 号：R735.7[医药卫生—肿瘤]