血管紧张素转换酶2在大鼠胰腺组织中的表达  

Expression of angiotensin-converting enzyme 2 in rat pancreas

作　　者：郭丽敏[1] 张雪莲[1] 杨金奎[1] 

机构地区：[1]首都医科大学北京同仁医院内分泌科,北京100730

出　　处：《基础医学与临床》2007年第5期490-494,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30671001)

摘　　要：目的检测SARS冠状病毒(SARS-CoV)S蛋白的功能性受体血管紧张素转换酶2(ACE2)在大鼠内脏尤其在胰腺内、外分泌腺的表达。方法麻醉Wistar大鼠后取胰、肺、肝、肾、心、肠、膀胱和脾8种组织,采用免疫组化和RT-PCR方法检测各组织ACE2的表达。结果ACE2在组织中均有不同程度的表达,其中胰腺外分泌腺ACE2阳性细胞的吸光度值较内分泌腺显著增加(P<0.01)。结论ACE2在大鼠胰腺内、外分泌腺的表达可介导SARS-CoV侵入并损害胰腺,使胰岛素耗竭,引起血糖水平升高;同时ACE2在内脏组织的普遍表达为SARS-CoV的入侵提供了可能。Objective To investigate the expression of angiotensin-converting enzyme 2 （ACE2） in rat endocrine and exocrine tissue of pancreas and to compare its expression level with that in other visceral tissues. Methods The expression of ACE2 in rat visceral organs was detected by immunohistochemistry and reverse transcriptase poly- merase chain reaction （RT-PCR）. Results ACE2 was positively expressed in all the test tissues, including pancreas, heart, liver, kidney, lung, spleen, duodenum, ileum and bladder. The expression of ACE2 mRNA was detected in whole pancreas and ACE2 protein was found in both endocrine tissue and exocrine tissue of rat pancreas, while in the endocrine tissue much less ACE2 protein was expressed. The absorbance value of exocrine tissue of rat pancreas increased significantly as compared to that in pancreatic islet. Conclusion Our data demonstrated a tissue specific pattern of ACE2 expression in rat pancreas and this indicated ACE2 might be present in islet of pancreas of human, which might cause the elevation of blood glucose levels in SARS patients. Since ACE2 works as a negative regulator of the angiotensin system, the expression of ACE2 protein in endocrine tissue of pancreas may facilitate the treatment of diabetes.

关 键 词：血管紧张素转换酶2 SARS冠状病毒 S蛋白 胰腺 

分 类 号：R576[医药卫生—消化系统]