基因枪转导K-RAS反义基因对胰腺癌细胞(K-RAS P21)蛋白表达的影响  

Effect of gene gun transduction of K-RAS Antisense gene on the expression of K-RAS P21 protein in human pancreatic carcinoma cells

作　　者：李晓冬[1] 张峰[2] 马毓梅[3] 蔡建辉[2] 

机构地区：[1]河北医科大学第二医院妇产科,河北石家庄050000 [2]河北医科大学第二医院外科,河北石家庄050000 [3]河北医科大学第二医院病理科,河北石家庄050000

出　　处：《基础医学与临床》2007年第5期495-498,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30140008)

摘　　要：目的观察基因枪转导K-RAS突变特异性反义基因对胰腺癌细胞K-RAS P21蛋白的影响。方法利用基因枪技术,将反义基因转导入宿主细胞,通过免疫细胞化学和Western blot方法,观察K-RAS突变特异性反义基因转导后AsPC-1、MiaPaCa-2和BxPC-3三种人胰腺癌细胞系K-RAS P21蛋白的变化。结果转导K-RAS突变特异性反义基因后,AsPC-1和MiaPaCa-2胰腺癌细胞的K-RAS P21蛋白表达明显降低;BxPC-3胰腺癌细胞的K-RAS P21蛋白表达无明显变化。结论突变特异性K-RAS反义基因经基因枪有效转导后,可用于胰腺癌的治疗。Objective To observe the effect of gene gun transduction of K-RAS Antisense gene on the expression of K-RAS P21 protein in human pancreatic carcinoma cells. Methods K-RAS Antisense gene was transduced into pancreatic carcinoma cells by gene gun transduction, the expression of K-RAS P21 protein on BxPC-3 ,AsPC-1 and MiaPaCa-2 pancreatic carcinoma cells line were examined by western blot and immunocytochemistry staining. Results The expression of K-RAS P21 protein in AsPC-1 and MiaPaCa-2 pancreatic carcinoma cells was obviously lower after the transduction of specific K-RAS Antisense gene ,which has little impact on BxPC-3 pancreatic carcinoma cells. Condusion Gene gun transduction of K-RAS Antisense gene is a potential method for treatment of pancreatie carcinoma.

关 键 词：胰腺癌 基因枪 基因治疗 反义基因 

分 类 号：R735.9[医药卫生—肿瘤]