食管上皮癌变过程中FAS、FASL、FADD和caspase-8的表达及其意义  

Expressions in FAS、FASL、FADD、 caspase-8 and their significant in esophageal carsinogenesis

作　　者：许欣[1] 彭林涛[1] 刘丽华[1] 王佩[1] 左连富[2] 

机构地区：[1]河北医科大学病理学教研室,河北石家庄050017 [2]河北医科大学第四医院肿瘤研究所,河北石家庄050011

出　　处：《基础医学与临床》2007年第5期525-529,共5页Basic and Clinical Medicine

摘　　要：目的研究细胞凋亡调控因子FAS、FASL、FADD和caspase-8基因在食管上皮癌变过程中的表达及其意义。方法用免疫组化SP法检测食管黏膜中FAS、FASL、FADD和caspase-8的表达;原位杂交法检测食管黏膜中FADDmRNA和caspase-8mRNA的表达。结果从食管正常黏膜组织到不典型增生和食管癌组织,FAS蛋白表达率呈逐渐下降趋势,FASL蛋白表达率呈逐渐上升趋势,食管癌与正常组织之间差异明显(P<0·05)。高、中分化鳞癌中蛋白阳性表达率显著高于低分化鳞癌(P<0·05)。从食管正常黏膜组织到不典型增生和食管癌组织,FADD和caspase-8蛋白及RNA阳性表达率呈逐渐下降趋势,食管癌与正常黏膜组织之间差异明显(P<0·01);caspase-8蛋白和mRNA在高、中分化鳞癌中的表达率高于低分化鳞癌(P<0·05)。结论FAS与FASL的表达失衡与食管癌的发生、发展有密切关系,并与食管癌的分化和免疫逃避有关。FADD与caspase-8基因表达异常在食管癌的发生、发展中可能起着重要作用。Objective To investigate the effects of the gene expressions of FAS, FASL, FADD and caspase-8 in esophageal carsinogenesis. Methods Immunohistochemical method was applied to detect the expression of FAS, FASL,FADD and caspase-8 proteins in esophageal epithelium. In situ hybridization method was applied to detect the expression of FADD and caspase-8 mRNA in esophageal epithelium. Results The positive rates of FAS, FADD and caspase-8 proteins and FADD,caspase-8 mRNA were decreased from normal epithelium to dysplasia and carcinoma tissues gradually. The positive rates of FASL protein were increased from normal epithelium to dysplasia and carcinoma tissues gradually. There was very significant statistical difference between carcinoma and normal epitheli- um（P 〈0. 05）. The expression of FAS,FASL and caspase-8 was correlated with the degree of tumor differentiation （ P 〈 0. 05）. Conclusion The abnormal expression of FAS and FASL maybe relate with the pathogenesis of esophageal carcinoma and is also involved in the degree of differentiation and the immune escape. FADD and caspase-8 expression maybe plays an important role in esophageal carsinogenesis.

关 键 词：食管癌 细胞凋亡 FAS/FASL FADD CASPASE-8 

分 类 号：R735.1[医药卫生—肿瘤]