烟草内生短短芽孢杆菌的分离鉴定及对烟草青枯病的防效  

Isolation and identification of endophytic Brevibacillus brevis and its biocontrol effect against tobacco bacterial wilt

作　　者：易有金[1] 尹华群[2] 罗宽[1] 刘学端[2] 刘二明[1] 

机构地区：[1]湖南农业大学生物安全科技学院,长沙410128 [2]中南大学资源加工与生物工程学院,长沙410083

出　　处：《植物病理学报》2007年第3期301-306,共6页Acta Phytopathologica Sinica

基　　金：湖南省教育厅重点资助项目(02A019)

摘　　要：在烟草青枯病区采取健康烟草植株,从其茎杆内分离到2株对烟草青枯拉尔氏菌(Ralstonia solanacarum)有强拮抗作用的内生菌株009和011。形态观察、生理生化鉴定及16S rDNA序列比对结果表明,菌株009和011均归属为Brevi-bacillus brevis,009、011菌株与B. brevis(AY591911)相似性分别为99.5%和99.0%,GenBank登录号分别为DQ444284、DQ444285。生长特性研究结果表明,它们的最适生长pH值分别为6.5、7.5,最适生长温度分别为25、30℃。温室内用淋根法分别先接种009和011菌株,后接种病原菌,其防效分别为87.25%和52.30%。用009和011菌液分别和烟草青枯病菌的混合液淋根,其防效明显低于前者。田间小区试验结果表明,011菌株的防效明显高于009菌株和农用链霉素。Endophytic bacterial strains 009 and 011 isolated from the stems of the health tobacco growing at infected fields strongly inhibited growth of Ralstonia solanacarum, the pathogen of tobacco bacterial wilt. Based on morphological, physiological, biochemical characteristics, and 16S rDNA phylogenetic analysis, strains 009 and 011 were identified as Brevibacillus brevis. High similarities of 16S rDNA sequence between strains 009 and 011with B. brevis （AY591911） were 99.5% and 99.0% respectively. The GenBank accession numbers of are DQ444284 for strain 009 and DQ444285 for strain 011. The optimal pH and temperature for the growth of 009 and 011 were 6.5 and 7.5,25 and 30℃ respectively. Greenhouse tests showed that 87.25% and 52.30% of control effects on tobacco bacterial wilt were obtained when strains 009,011 were respectively watered tobacco roots in advance of inoculating R. solanacarum. However, if strain 009 and strain 011 were watered tobacco roots simultaneously with R. solanacarum, the treatments of strains 009 and 011 were lower effective than the former. The control effect of strain 011 against the disease was superior to strain 009 and Streptomycini in the field.

关 键 词：烟草 内生细菌 短短芽孢杆菌 青枯拉尔氏菌 鉴定 防治 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]