含IKVAV肽体内诱导血管生成的实验研究  

Angiogenesis Induced with IKVAV-containing Peptide in vivo

作　　者：肖锋[1] 吴永超[2] 郑启新[2] 宋玉林[2] 

机构地区：[1]湖北省荆州市第一人民医院,湖北荆州434000 [2]华中科技大学同济医学院附属协和医院骨科,湖北武汉430022

出　　处：《生物骨科材料与临床研究》2007年第1期5-7,共3页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：国家自然科学基金(30500511)

摘　　要：目的探讨含IKVAV肽体内自组装成凝胶材料诱导血管生成的可行性。方法实验组:配制1%的肽溶液,pH=9．0。对照组:配制16．67%的明胶,pH=7．4。各取1毫升,分别注射植入大鼠背部脊柱两侧皮下。观察植入后一周内鼠的全身反应及植入部位的皮肤变化。结果二周后取材,固定,常规HE染色及VEGF免疫组织化学染色。术后一周实验动物存活良好,无全身不良反应,植入部位皮肤无红肿、无坏死。实验组:植入点的1%的肽自组装成凝胶,HE染色显示,凝胶与周围组织间整合好,无炎性细胞浸润,周围有血管长入凝胶内部,血管腔内可发现红细胞,术后两周,VEGF免疫组织化学染色阳性。对照组:HE染色,明胶与组织的相容性好,在明胶内未发现血管;VEGF免疫组织化学染色阴性。结论含IKVAV肽体内可自组装成凝胶,并且可诱导新生血管在凝胶内生成。Objective To explore the feasibility of angiogenesis induced with IKVAV-containing peptide self-assembly into hydrogel in vivo. Methods 1% peptide solution where pH was equal to 9.0 was prepared in experiment group （EG）. 16. 67% gelatin solution in which pH was identical to 7.4 was also prepared in control group （EG）. 1 millilitre of 1% peptide solution and that of 16. 67 % gelatin solution were injected subcutaneously beside rat backbones. The systemic response and local skin change were observed in one week of post-injection. Results Two weeks later, the peptide hydrogel and gelatin included in the peripheral organs were harvested and fixed, and stained with HE and identified with immunohistochemistry VEGF. In one week of post-implantation, the rats all lived well without generally adverse reaction. There were no flare and necrosis of local skins. 1% peptide solution was self-assembly into hydrogel in subcutaneous. HE showed that Hydrogel was integrated well with organs without inflammatory cell infiltration, the capillaries where the erythrocytes appeared sprouted into thehydrogel. Aftertwoweeksofpost-operation, immunohistochemistryVEGFwaspositive. The gelatin, where the capillaries were not found, was compatible to the organs, and immunohistochemistry VEGF was negative. Conclusion IKVAV-containing peptide was self-supported into hydrogel and induced the angiogenesis in vivo.

关 键 词：血管生成 自组装 凝胶 IKVAV 

分 类 号：R318.0[医药卫生—生物医学工程]