Kai-1基因外显子缺失与大肠癌的临床关系  

Relationship between loss of exon 9 of Kai-1 gene and clinicopathological significance in colorectal cancer

作　　者：陈刚[1] 陈森清 张晓梅[1] 尚俊清[1] 周建农[1] 

机构地区：[1]南京医科大学附属江苏省肿瘤医院普外科结直肠外科组,江苏南京210009 [2]江苏省肿瘤研究所分子生物室,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2007年第6期594-597,F0004,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅自然科学基金重点资助项目(H2009)

摘　　要：目的:探讨Kai-1基因的缺失在结直肠癌演进与转移中的意义。方法:提取40例结直肠癌患者的肿瘤组织(24例无淋巴转移,16例有淋巴转移),15例癌旁正常组织的RNA,RT-PCR扩增,电泳检测,测序验证Kai-1基因的缺失情况。结果:40例组织标本中18例出现Kai-1基因exon9缺失(16例杂合缺失,2例纯合缺失),15例癌旁正常组织中有2例出现Kai-1基因exon9缺失(2例杂合缺失);在大肠癌组织中Kai-1基因缺失频率显著高于在癌旁正常组织中(P<0.05);在有淋巴转移的结直肠癌组织中,缺失的频率明显高于无淋巴转移的结直肠癌组织(P<0.05),在大肠癌中晚期(DukesC期)明显高于早期(DukesA-B期)(P<0.05),而Kai-1基因的缺失频率与结直肠癌患者的年龄、性别、组织学类型以及分化程度无关(P>0.05)。结论:Kai-1基因缺失可能与在大肠癌演进、转移有关,检测其缺失可作为判断大肠癌的演进与转移的客观临床指标。Objective：To explore the significance loss of Kai-1 gene plays in the progression and metastasis of colorectal cancer （CRC）. Methods：The deletion of Kai-1 gene were detected by using RT-PCR and validated by sequencing in the tumor of 40 cases of CRC and 15 normal cases near the tissues of CRC. Results：The deletion of exon 9 in the Kai-1 gene was found in 18 of 40 colorectal cases （45%） and in 2 of 15 normal cases near the colorectal tissues （13.3%）. The frequency of the exon 9 deletion was significantly higher in the colorectal tumors than in the normal cases （P 〈 0.05）. The frequency of the exon 9 deletion was significantly higher in the colorectal tumors with lymphatic metastasis than that without lymphatic metastasis （P 〈 0.05）, and in the advanced stage （Dukes C,D） than in the earlier stage （Dukes A,B） of the colorectal cancer （P 〈 0.05）. However, there was no significant difference of the frequency of exon 9 deletion when stratefied by the the age, sex, histological type as well as differentiated degree of the patients with CRC （P 〉 0.05）. Conclusion：The deletion of the Kai-1 was associated with the progression and metastasis of CRC, and the detection of the deletion of the Kai-1 can be considered as an objective clinical marker to assess the progression and metastasis of CRC.

关 键 词：大肠肿瘤 KAI-1基因 缺失 转移 

分 类 号：R735.34[医药卫生—肿瘤]