脂多糖诱导急性肺损伤过程中SSAO酶的活性变化  

Changes of Semicarbazide-sensitive Amine Oxidase (SSAO) Activity in Pulmonary Inflammation Induced by Lipopolysaccharide

作　　者：张美[1] 李慧[1] 罗红军[1] 罗文鸿[1] 

机构地区：[1]汕头大学医学院中心实验室,广东汕头515041

出　　处：《癌变．畸变．突变》2007年第3期223-226,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(No.30271139);广东省自然科学基金(No.022124);2003年度教育部"优秀青年教师资助计划"项目(教人司[2003]355号)

摘　　要：背景与目的:探讨SSAO酶在脂多糖诱导急性肺损伤过程中的活性变化。材料与方法:将15只雄性成年新西兰兔随机分为模型A组、模型B组和对照组,每组5只。模型组通过气管插管直接给予脂多糖2mg/kg,其中A组在给药后48h与对照组均用于制作肺组织HE切片;B组用于动物体征观察、外周血白细胞计数和SSAO酶活性检测,并对给药前、后各指标的变化进行比较。结果:模型组给药后外周血白细胞数目和肺组织HE染色等炎症指标与对照相比均有明显变化,SSAO酶活性在给药后的16h酶活性迅速上升至最高,然后在48h逐渐下降至最低点,其升高或降低幅度,较给药前均有统计意义(P<0.05或P<0.01)。结论:采用气管直接插管法给予脂多糖的造模方法稳定可靠,在产生急性肺损伤过程中会导致外周血SSAO酶活性明显的规律性波动。BACKGROUND ＆ AIM： To investigate the changes of semicarbazide- sensitive amine oxidase（SSAO） activity in lipopolysaccharide-induced pulmonary inflammation. MATERIALS AND METHODS： 15 adult male New Zealand white rabbits were randomly divided into three groups with 5 rabbits in each, two model groups （A and B） and one negative control group.The rabbit pulmonary inflammation model was developed by inhalation of lipopolysaccharide （2 mg/kg）. Group A and the control were sacrificed after 48 hours, HE-stained sections of the lung were examined for histopathological changes.Group B was monitored by clinical features, WBC, and the fluctuation of plasma SSAO activity was measured and a comparison was made on the indexes before and after the treatment with LPS. RESULTS： Based on the number of WBC in peripheral blood and lung tissue HE stain after the treatment, LPS induced significant acute lung injury. The SSAO activity fluctuated and correlated with pathological change. After the treatment, the SSAO activity peaked at 16 hour and then decreased to the lowest level at 48 hour. The peak and trough activities were significant ly/ different compared to 0 hour before treatment（P〈0.05 or P〈0.01） . CONCLUSION： LPS administered through trachea cannula could reliably lead to acute lung injury and induce regular fluctuation of plasma SSAO activity.

关 键 词：脂多糖 肺炎模型 氨基脲敏感胺氧化酶 

分 类 号：R364.5[医药卫生—病理学]