MRK-16修饰阿霉素免疫脂质体逆转肺癌细胞多药耐药研究  

ADM-loaded immunoliposomes reverse MDR of human lung carcinoma in vitro

作　　者：叶果[1] 柯爱武[2] 李羲[3] 

机构地区：[1]江苏省常熟市第二人民医院呼吸内科,江苏常熟215500 [2]复旦大学中山医院,上海200032 [3]海南医学院附属医院呼吸内科,海南海口570228

出　　处：《现代肿瘤医学》2007年第6期754-757,共4页Journal of Modern Oncology

摘　　要：目的:研究MRK-16修饰阿霉素免疫脂质体与阿霉素脂质体对非小细胞肺癌多药耐药的逆转作用。方法:25%的戊二醛偶联MRK-16于阿霉素脂质体,制备MRK-16修饰阿霉素免疫脂质体;以MTS测定细胞存活率,检测SPC-A1/TAX的耐阿霉素指数;以阿霉素脂质体与MRK-16修饰阿霉素免疫脂质体作用于SPC-A1/TAX,设阿霉素组对照;研究两者对SPC-A1/TAX的体外细胞毒与多药耐药逆转作用;流式细胞仪检测用药后2、4、6小时后细胞内药物浓度。结果:免疫脂质体细胞杀伤率(48h)达67.7%,其多药耐药逆转指数达7.45;脂质体对细胞的杀伤率49.2%,逆转指数4.28;流式细胞仪检测细胞内药物浓度,6小时后阿霉素免疫脂质体组细胞内药物浓度是阿霉素组的18.34倍,脂质体组是阿霉素的10.1倍。结论:MRK-16修饰阿霉素免疫脂质体、阿霉素脂质体在体外对非小细胞肺癌多药耐药具有一定的逆转作用。Objective ： To determine the reversion of MDR of human lung carcinoma by ADM liposomes and ADM immunoliposomes modified by MRK - 16 in vitro. Methods. Liposomes were made into immunoliposomes by conjugating monoclonal antibodies through glutaraldehyde, the study of cytotoxocity of ADMILs, ADMLs on SPC - A1/TAX cell lines and reversion of MDR were determined by measuring the inhibition of cell growth using the MTS assay, and the amount of intracellular ADM accumulation was determined by flow cytometry. Results ：The death rate of targeting cells after ADMILs acting for 48 hours was 67.7 percent, the toxicity of ADMILs to cancer cells was 7.25 times than ADM, and the ADMLs was 49.2% and 4.28. The increased sensitivity of MDR cells to ADMILs and ADMLs was accounted for by a remarkable increase intracellular ADM accumulation which was 18.34 and 10.1 times than ADM acting. Conclusion：The liposomes and immunoliposomes bearing MRK - 16 are effective to reverse MDR of human lung carcinoma in vitro.

关 键 词：阿霉素 脂质体 免疫脂质体 SPC—A1/TAX 多药耐药逆转 

分 类 号：R734.2[医药卫生—肿瘤]