RegⅣ基因在大肠癌中抗凋亡作用的体外研究  

Study on anti-apoptosis effects of RegⅣ gene in colorectal cancer in vitro

作　　者：郑声琴[1] 何杰[1] 王建华[1] 黄培林[2] 高峰[3] 

机构地区：[1]东南大学附属中大医院病理科,江苏南京210009 [2]东南大学基础医学院病理学与病理生理学系,江苏南京210009 [3]东南大学临床医学院中心实验室,江苏南京210009

出　　处：《现代医学》2007年第3期183-186,共4页Modern Medical Journal

基　　金：国家自然科学基金资助项目(30400534);江苏省青年科技创新人才项目(2004407)

摘　　要：目的观察RegⅣ基因在大肠癌细胞系中的表达情况及其体外抗凋亡作用。方法采用RT-PCR和免疫组织化学染色法检测4种大肠癌细胞系中RegⅣ基因的表达,通过MTT法分析不同浓度的姜黄素对4种大肠癌细胞系(HT-29、Caco2、Sw480和Lovo)的生长抑制作用,应用流式细胞仪评价RegⅣ基因的抗凋亡作用。结果RegⅣmRNA在HT-29细胞系高表达,Caco2细胞系较高表达,Sw480细胞系较低表达,Lovo细胞系不表达;RegⅣ蛋白在HT-29细胞胞核和胞质内表达阳性,Lovo细胞胞核和胞质表达阴性,Sw480细胞胞质内表达阳性,Caco2细胞胞质内表达弱阳性。不同浓度的姜黄素对4种细胞系的生长抑制作用强弱依次为Lovo>Sw480>Caco2>HT-29;用20μmol.L-1的姜黄素处理24 h后,4种细胞系的凋亡率大小依次为Lovo>Sw480>Caco2>HT-29。结论RegⅣ基因高表达与大肠癌发生可能有关;RegⅣ基因在大肠癌中可能具有抗凋亡作用。Objective To investigate the expression and anti-apoptosis effects of Reg Ⅳ gene in colorectal cancer cell lines. Methods The expression of Regiv gene was detected by RT-PCR and immunohistochemistry in 4 colorectal cancer cell lines （ HT-29, Caco2, Sw480 and Lovo）. MTF assay was used to evaluate the cytotoxicity of curcumin in 4 colorectal cancer cell lines. Anti-apoptosis effects of Regiv gene was detected by flow cytometry. Results There was higher expression of Regiv mRNA in HT-29, high in Caco2 and less in Sw480, and noexpression in Lovo. There was higher expression of Regiv protein in the cytoplasm and nucleolus of HT-29 ,high expression in the cytoplasm of Sw480 and lower expression in Caco2 ,but it wasn＇t found in Lovo. Different concertrations of curcumin inhabited growth of the cells in turns ： Lovo 〉 Sw480 〉 Caco2 〉 HT-29. After 24 h under 20 mmol·L^-1 of curcumin, the turns of the ratio of apoptosis was Lovo 〉 Sw480 〉 Caco2 〉 HT-29. Conclusion The high expression of Regiv may be concern with the tumorigenesis of colorectal cancer. Regiv gene may play an anti-apoptosis role in colorectal cancer cell lines.

关 键 词：大肠癌 RegⅣ基因 细胞培养 免疫组织化学 抗凋亡 

分 类 号：R735.34[医药卫生—肿瘤] Q786[医药卫生—临床医学]