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作 者:潘力[1] 黄耀威[1] 叶燕锐[1] 王亚琴[1]
机构地区:[1]华南理工大学生物科学与工程学院,广州510641
出 处:《微生物学报》2007年第3期517-521,共5页Acta Microbiologica Sinica
基 金:广东省自然科学基金项目(04020061)~~
摘 要:根据嗜杀酵母T158c/S14a中L-A病毒-1移码效率改变影响M1病毒的存活,导致K1毒素减少,在低pH的美蓝平板上用杯碟法通过抑菌圈的大小检测酵母K1毒素的嗜杀活性,建立了一个以酵母嗜杀系统为基础的抗病毒药物筛选模型.研究了杯碟法检测酵母毒素嗜杀活性的各种条件.对不同pH和温度下酵母的嗜杀活性进行了研究,确定了模型用于筛选的最适pH范围为4.3~4.7,最适温度范围为20~22℃.运用该模型研究了几种中药对嗜杀活性的抑制作用,发现了金银花和升麻具有一定的抗病毒作用.该模型为抗病毒药物的高通量初筛奠定了基础.Altering -1 frameshifting efficiency of L-A virus in yeast T158c/S14a will result in loss of M1 virus and reduction of toxin K1, which induced a diminished of inhibition zone. According to the size of inhibition zone on methylene blue flat with low pH, a model for screening anti-viral agents was developed. The conditions of assaying killing activity by well test assay were explored. With assaying the killing activity in different pH and temperature, it was found that the optimum pH range of 4.3 - 4.7 and the optimum temperature range of 20 - 22 ℃ in this screening model. To utilize this screening model, Several Chinese crude drugs had been studied and were found Flos Lonicerae and Rhizoma Cimicifugae have anti-viral effect. Such a model was to establish foundation for high throughput screening of anti-viral agents.
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