气单胞菌几丁质酶的产酶条件优化研究  

Condition Optimization for Chitinase Produced from Aeromonas sp.

作　　者：张莉[1] 杨土凤[1] 陈晓梅[1] 张海英[1] 徐敏[1] 刘世贵[1] 龙章富[1] 

机构地区：[1]生物资源与生态环境教育部重点实验室四川大学生命科学学院,四川成都610065

出　　处：《安徽农业科学》2007年第17期5035-5037,5039,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为了进行气单胞菌几丁质酶产酶条件的研究。[方法]从自然死亡家蚕中肠围食膜中分离的气单胞菌进行产酶优化试验。其优化条件为:粉状几丁质1.2%,K2HPO4 0.12%,乙酸铵0.02%,发酵72 h,温度为30℃,起始pH值为8.0,培养基装量为15%。[结果]结果表明:该菌株所产几丁质酶的最适反应温度为25℃,pH值5.5,耐温性较差,但pH值5.0～9.0的酶活力较稳定(>88%)。[结论]该研究提供了提高几丁质酶产量的途径,但对该菌株进行基因改造或诱变育种,充分发掘其产酶潜力,对其几丁质酶基因进行克隆、表达等仍需进一步研究。[Objective] The aim of this paper was to study the condition for chitinase produced from A eromonas sp. [Method] A eromonas sp. was isolated from Bombyx mori. And its condition to produce chitinase was studied, which were 1.2 % powder chitin, 0.12 % K2HPO4, 0.02 % ammonium acetate, 8.0 initial pH, 15 % culture medium volume, 72 h culture time and inoculated in 30℃. [Result] Result showed that the optimum condition forAeromonas sp. to produce chitinase were 25℃ and pH 5.5. It was poor in heat resistance but was stable at pH 5.0-9.0 with at least 88 % activity. [Conclusion] Approaches to improve chitinase yield were provided in this paper. But there still needed further study in gene modification, mutation breeding, tapping the enzyme-producing potential, chitinase gene cloning and expression.

关 键 词：发酵优化 均与设计 几丁质酶 性质 

分 类 号：Q814[生物学—生物工程]