宫颈癌患者血液中人乳头瘤病毒检出率的临床意义  

Clinical significance of plasma HPV-16 and HPV-18 DNA detection in patients with cervical cancer

作　　者：王冬冬[1] 林蓓[1] 周丹[1] 张宇华[1] 董伟妍[1] 齐跃[1] 张帆[1] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004

出　　处：《中国医科大学学报》2007年第3期330-331,共2页Journal of China Medical University

基　　金：辽宁省科技攻关项目(2005225007-2);沈阳市科技攻关项目(1053125-54-1)

摘　　要：目的:探讨宫颈癌患者血液中人乳头瘤病毒(HPV)DNA检出率对宫颈癌的诊断及病情进展评价的临床意义。方法:应用巢式PCR检测48例宫颈癌,55例宫颈高度病变(HG),6例宫颈低度病变(LG),29例宫颈炎症患者血浆中HPV-16,18DNA。分析各组患者血中HPVDNA检出率的差异,以及检出率与宫颈癌临床分期的相关性。结果:血浆中HPVDNA在宫颈癌组、HG组、LG组及宫颈炎症组中的检出率分别为56.3%、41.8%、16.7%和37.9%,其中HPV-16分别为20.8%、5.5%、0.0%和6.9%;HPV-18分别为50.0%、38.2%、16.7%和31.0%。HPV-16和HPV-18均为阳性者8例,其中宫颈癌Ⅰ期3例,Ⅱ期4例,CINⅢ1例。在9例有淋巴结转移的患者中,6例血浆HPVDNA阳性。血浆HPVDNA在宫颈癌组中检出率高于其他3组,但无统计学差异。结论:宫颈癌患者血中HPV检出率高于CIN和宫颈炎症患者,并且在发生转移的宫颈癌患者中检出率更高,但血浆HPVDNA检出率与宫颈癌临床分期无关。宫颈癌患者血中HPV双重感染率较高。Objective： To explore the clinical significance of the detection of plasma human papillomavirus（HPV） DNA in evaluating the diagnosis and progression of cervical cancer, Methods： Nested polymerase chain reaction was performed to detect plasma HPV-16 and HPV-18 DNA in 48 patients with cervical cancer, 55 with high-grade cervical lesion, 6 with low-grade cervical lesion, and 29 with infective cervical lesion. The correlation between plasma HPV-16 and HPV-18 DNA and the clinical stage of cervical cancer was analyzed by Chi-square test. Results： The detection rates of plasma HPV DNA were 56.3%, 41.8%, 16.7% , and 37.9% in patients with cervical cancer, high-grade cervical lesion, low-grade cervical lesion, and infective cervical lesion, respectively. The detection rate of plasma HPV DNA in patients with cervical cancer was higher than that in patients of other 3 groups, but the difference was not significant. There were 8 patients in whom both plasma HPV-16 and HPV-18 DNA were positive. Plasma HPV DNA was detected in 6 of 9 patients with pelvic lymph node metastasis. There was no correlation between the detection rate of plasma HPV DNA and the clinical stages of cervical cancer. Conclusion： The detection rate of plasma HPV DNA is higher in patients with cervical cancer, especially in those with lymph node metastasis. Double infection of HPV-16 and HPV-18 is more common in patients with cervical cancer.

关 键 词：宫颈癌 人乳头瘤病毒 巢式PCR 血浆 

分 类 号：R737.33[医药卫生—肿瘤]