γ-干扰素、全反式维A酸对皮肤鳞状细胞癌SCL-1细胞表达生存素的影响  

Effects of all-trans-retinoic acid andγ-interferon on the expression of survivin in SCL-1 cells

作　　者：王珍[1] 何春涤[1] 金鑫[1] 肖汀[1] 王雅坤[1] 金光玉[1] 陈江[1] 姜奕[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科,沈阳110001

出　　处：《中华皮肤科杂志》2007年第6期365-367,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(30271198);教育部留学回国人员科研启动基金[2002]247号

摘　　要：目的探讨γ-干扰素、全反式维A酸(ATRA)对皮肤鳞状细胞癌细胞株(SCL-1)表达生存素的影响。方法采用RT-PCR、免疫印迹(Western blot)分析方法分别检测γ-IFN、ATRA作用前后SCL- 1细胞株生存素mRNA及蛋白表达的差异。结果γ-IFN处理后,生存素在SCL-1细胞表达较对照组明显下降(P<0.05),且具有时间依赖性和剂量依赖性;ATRA可以增强γ-IFN对生存素的抑制作用。结论γ-IFN联合ATRA诱导皮肤鳞状细胞癌细胞发生细胞凋亡的机制之一可能与下调生存素的表达相关。Objective To study the effects of all-trans-retinoic acid （ATRA） and γ-interferon （ γ-IFN ） on the expression of survivin in skin squamous cell carcinoma cell line SCL-1. Methods SCL-1 cells were treated with γ-IFN alone （ 100, 500, 1000 u/mL, respectively ） or in combination with ATRA （ 1000 u/mL γ-IFN ＋ 10^6 mol/mL ATRA ） in vitro. The mRNA and protein expressions of survivin were detected in SCL-1 cells by RT-PCR and Western-blot respectively. Results γ-IFN could significantly inhibit the mRNA and protein expressions of survivin in a dose- and time,dependent manner. Also, ATRA could enhance the inhibition of survivin expression by γ-IFN. Conclusion The down-regulation of survivin expression may be a mechanism for the induction of apoptosis of skin squamous cell carcinoma cells by the combination of γ-IFN and ATRA.

关 键 词：癌.鳞状细胞 维生素A酸类 干扰素Ⅱ型 生存素 细胞系 肿瘤 

分 类 号：R739.5[医药卫生—肿瘤]