氯仿-甲醇提取贝氏柯克斯体残存组分Q热疫苗的免疫原性及免疫保护性研究  

Immunogenicity and immnoprotectivity of Q fever vaccine of chloroform-methanol residue of Coxiella burnetii

作　　者：孙长俭[1] 陈梅玲[1] 杨晓[1] 温博海[1] 牛东升[1] 李青凤[1] 王锡乐[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《中国人兽共患病学报》2007年第6期544-547,共4页Chinese Journal of Zoonoses

基　　金：国家863计划项目(2006AA02Z459)资助

摘　　要：目的实验评价氯仿-甲醇提取贝氏柯克斯体残存组分(CMR)疫苗的免疫特性及免疫保护性。方法采用国内分离贝氏柯克斯体新桥株制备CMR疫苗,用CMR免疫Balb/c小鼠;采用间接免疫荧光法检测CMR免疫小鼠血清特异性抗体水平和用淋巴细胞增殖试验评价CMR疫苗体外刺激小鼠脾细胞增殖的能力;以贝氏柯克斯体攻击免疫小鼠和用贝氏柯克斯体特异的荧光定量PCR检测感染小鼠血和脾脏样本。结果CMR免疫第4周起,小鼠血清中检出高水平的特异性抗体,CMR加氢氧化铝佐剂免疫小鼠的血清特异性抗体显著高于未加佐剂组。CMR在体外刺激正常小鼠和免疫小鼠脾淋巴细胞增殖水平显著高于对照组,WCV则抑制正常脾淋巴细胞增殖。三种剂量(30μg、10μg、1μg/只)CMR免疫小鼠血和脾脏样本贝氏柯克斯体含量显著低于未免疫组,以30μg组及加佐剂免疫小鼠的样本含菌量更显著低于对照组。结论采用我国分离株制备的CMR疫苗能诱导机体产生高效特异性体液免疫和细胞免疫应答,使机体抵抗大剂量的贝氏柯克斯体感染,具有良好的免疫原性和免疫保护性;氢氧化铝佐剂能显著增强CMR疫苗的免疫保护效能。To investigate the immunogenieity and immunoproteetivity of the chloroform-methanol residue （CMR） vaccine of Coxiella burnetii. The CMR vaccine was prepared with C. burnetii Xinqiao strain isolated in China. Balb/c mice were immunized with CMR, and the specific antibody level of the immunized mice was analyzed by indirect immunofluoresee assay and cellmediated immunity response was detected by assay of the proliferation of the lymphocyte in vitro. The pathogenic agent in the blood and spleen samples of the mice attacked by C. burnetii was detected by quantitative real-time PCR specific for the agent. The high levels of specific antibody （IgG） to C. burnetii and proliferation of lymphoeytes responding to CMR were detected in sera and splenic cells from the mice immunized , respectively. The blood and spleen samples from mice immunized with three dosages of CMR（30μg, 10μg, or 1μg per mouse）were detected in much lower levels of C. burnetii compared with that from mice without immunization and the quantity of C. burnetii in the samples from the mice immunized with CMR absorbed with Al（OH）3 were significantly lower than that from mice immunized with CMR. CMR prepared with Xinqiao strain can efficiently induce protective specific humoral and cell-mediated immune responses against infection of C. burnetii. The adjuvant Al（OH）3 can efficiently enhance the immunoproteetivity of CMR. CMR might be a good vaccine candidate against C. burnetii.

关 键 词：贝氏柯克斯体 Q热疫苗 氯仿-甲醇提取残存组分 

分 类 号：R378[医药卫生—病原生物学]