扇贝裙边糖胺聚糖对血管内皮细胞的抗氧化活性  

Antioxidative effect of scallop skirt-glycosaminoglycan on vascular endothelial cell

作　　者：高莹[1] 刘赛[1] 王海桃[1] 刑军[2] 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]莱芜市人民医院,山东莱芜271100

出　　处：《中国医院药学杂志》2007年第6期712-714,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:A30171157)

摘　　要：目的:探讨扇贝裙边糖胺聚糖(SS-GAG)的抗氧化活性。方法:用体外培养的人脐静脉内皮细胞株,建立过氧化氢(H2O2)诱导的血管内皮细胞损伤模型,用硫代巴比妥酸反应物法测定血管内皮细胞内氧化损伤产物丙二醛(MDA)的含量,用黄嘌呤氧化酶法测定细胞内的超氧化物歧化酶(SOD)的活性。结果:SS-GAG可明显减少MDA的生成,对细胞内的SOD活性有明显增强的作用。结论:SS-GAG通过拮抗H2O2对血管内皮细胞的过氧化损伤,发挥其抗氧化活性。OBJECTIVE To study antioxidative effect of scallop skirt-glycosaminoglycan （SS-GAG）. METHODS The endothelial cell strain of human umbilial vein （HUVEC, ECV304）had been cultured in vitro , and a model of endothelial cell oxidative damage induced by hydrogen peroxide（ H2O2 ） was established. TBARS method was used to analyse the level of malanyldiadeyhde （MDA） within cells and xanthine oxidase method was used to analysis the activity of superoxide dismutase （SOD）. RESULTS SS-GAG could decrease the level of MDA , promote the activity of SOD. CONCLUSION SS-GAG displays its oxidation resistance active through oppresses H2O2 to vascular endothelial cell peroxidation damage.

关 键 词：糖胺聚糖 丙二醛 超氧化物歧化酶 血管内皮细胞 抗氧化活性 

分 类 号：R285.6[医药卫生—中药学]