牛磺酸对大鼠移植胰腺缺血再灌注损伤的保护作用  

Protective effect of taurine against ischemia and reperfusion injury of transplanted pancreas in rats

作　　者：张克忠[1] 刘永锋[1] 程颖[1] 刘树荣[1] 王凤山[1] 石蕊[1] 赵宁[1] 

机构地区：[1]中国医科大学附属第一医院普外一科暨器官移植科,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2007年第12期1358-1363,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨牛磺酸在大鼠胰腺移植中对移植胰腺缺血再灌注损伤的保护作用及其机制.方法:采用大鼠胰十二指肠移植模型.Wistar大鼠30只随机分为三组(n=10):(1)对照组:CMU-1液保存6h再移植;(2)实验组A:CMU-1液+牛磺酸(5 mmol/L)保存6h再移植;(3)实验组B:CMU-1液+牛磺酸(10 mmol/L)保存6 h再移植(CMU-1液是本实验室配制的多器官保存液).于移植术后6h取静脉血作胰腺外分泌功能即血清淀粉酶测定,取胰腺组织作病理检查.通过免疫组化染色观察炎性细胞因子TNF-α及IL-1β表达情况.应用计算机图像分析系统测定其积分光密度值(IOD).结果:移植胰腺在通血后6h血清淀粉酶均升高,与对照组相比较,加入牛磺酸后淀粉酶升高较对照组降低(911.2±9.9,959.9±12.5 U/L vs 1239.5±68-3 U/L,P<0.05).随着牛磺酸浓度的增大其作用也显著.免疫组化结果显示,移植后6h胰腺组织TNF-α(12346.4±4933.69,16 016.33±4592.06 vs 22 039.71±4782.62,P<0.05及IL-1β(12 417.4±2222.9,1 5 047.68±4484.4 vs 19 284.46±3178.19.P<0.05)在各组均表达,但加入牛磺酸后能明显下调TNF-α及IL-1β的表达,各组内的差异具有显著性(P<0.05).光镜检查可见试验组的移植胰腺炎性改变明显减轻.结论:牛磺酸作为一种细胞保护剂能够抑制TNF-α和IL-1β的产生、抗细胞水肿,减轻胰腺缺血再灌注损伤,减轻胰腺炎症.AIM： To investigate the protective effect of taurine （TAU） on the ischemia and reperfusion injury during pancreas transplantation and its mechanism in rats. METHODS： Rat models of pancreatic-duodenal transplantation were used in this study. Thirty Wistar rats were randomly allocated into 3 groups （n = 10）, including control group （CMU-1 preservation solution）, experiment group A （CMU-1 ± TAU 5 mmol/L） and experiment group B （CMU-1 ± TAU 10 retool/L） （The liquid CMU-1 is multi-organ preservation solution produced in our laboratory）. Blood samples were collected 6 hours after reperfusion for examination of serum amylase level. Pancreatic tissues were collect for pathologic observation. The expression of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） were determined by immunohistochemistry using integrated optical density （IOD）. RESULTS： In comparison with that in control group, the level of serum amylase was increased 6 hours after reperfusion in experiment group A and B （911.2 ± 9.9, 959.9 ± 12.5 U/L vs 1239.5 ± 68.3 U/L, both P 〈 0.05）. The effect of TAU was enhanced with the increased of concentrations. Immunohistochemistry showed that both TNF-α and IL-1β were expressed in pancreatic tissues 6 hours after transplantation, and TAU significantly inhibited TNF-α （12 346.4 ± 4933.69, 16 016.33 ± 4592.06 vs 22 039.71 ± 4782.62, both P 〈 0.05） and IL-113 （12 417.4 ± 2222.9, 15 047.68 ± 4484.4 vs 19 284.46 ± 3178.19, both P 〈 0.05） expression in experiment group A and B. Taurine treatment markedly ameliorated the inflammatory reaction of pancreatic tissues. CONCLUSION： As a kind of cell-protection agent, TAU can inhibit the production of TNF-α and IL-1β, resist cell edema and alleviate ischemia/reperfusion-induced injury and pancreatitis.

关 键 词：牛磺酸 胰腺移植 缺血再灌注损伤 肿瘤坏死因子-α 白细胞介素-1Β 免疫组织化学 

分 类 号：R657.5[医药卫生—外科学]