层黏连蛋白对人翼状胬肉成纤维细胞生长的抑制作用  

The inhibition effects of laminin on human pterygium fibroblasts

作　　者：王欣玲[1] 于韬[2] 张国刚[3] 阎启昌[1] 张劲松[1] 

机构地区：[1]中国医科大学附属第四医院眼科,中国辽宁省沈阳市110005 [2]辽宁省肿瘤医院医学影像科,中国辽宁省沈阳市110042 [3]沈阳药科大学中药学院,中国辽宁省沈阳市110016

出　　处：《国际眼科杂志》2007年第3期645-647,共3页International Eye Science

基　　金：中国国务院第33批博士后科学基金资助项目(No.2003033282);辽宁省教育厅高等学校科学研究项目(No.2004C041);辽宁省教育厅高等学校科学技术研究项目(No.05L576);辽宁省科技厅博士启动;自然科学基金项目(No.20062091)~~

摘　　要：目的:探讨层黏连蛋白对体外培养的人翼状胬肉成纤维细胞增殖的影响。方法:体外培养的第3代翼状胬肉成纤维细胞,接种于层黏连蛋白包被培养皿(Ln组)和空白培养皿(对照组)中,倒置显微镜观察细胞生长状态,MTT法检测细胞增殖状态,作出生长曲线;免疫荧光法检测α-SMA在细胞内的表达;RT-PCR法检测TGF-β1和cytokeratin mRNA的表达水平。结果:Ln组成纤维细胞数量少于对照组;3～7d,Ln组A570明显低于对照组(0.20vs0.28,0.22vs0.30,0.28vs0.34,0.31vs0.36,0.33vs0.36,P<0.05);α-SMA在对照组细胞内表达良好,在Ln组的表达受到抑制;与对照组相比,Ln组TGF-β1mRNA的表达下降,cytokeratin mR-NA表达增加。结论:层黏连蛋白能抑制翼状胬肉中成纤维细胞的增殖。AIM： To observe the effects of laminin on pterygium fibroblasts proliferation in vitro. METHODS： The third passage subcultured pterygium fibroblasts were seeded in laminin-coated plates （In group） and blank plates （Control group）, The pterygium fibroblasts growth was observed with inverse microscopy and the images were recorded with digital camera. The cells proliferation was detected with MTT assay and the growth curve were made to show the difference trend （0.20 vs. 0.28, 0.22 vs. 0.30, 0.28 vs. 0.34, 0.31 vs. 0.36, 0.33 vs 0.36, P 〈0.05）. Positive expression of α-smooth muscle actin （α-SHA） was recorded with immunofluorescent microscopy. The mRNA level of TGF-β1 and cytokeratin were detected with reverse transcriptase-polymerase chin reaction （RT-PCR）. RESULTS： There were fewer cells on plates in in group than in control group. OD570 values were significantly lower during the 3-7 day in in group than the control group. The positive expressions of α-SMA were inhibited in I_n group, while they were quite well in the control group. The mRNA level of TGF-β1 decreased and that of cytokeratin increased in in group. CONCLUSION： Laminin can inhibit the proliferation of pterygium fibroblasts.

关 键 词：层黏连蛋白 翼状胬肉 成纤维细胞 

分 类 号：R777.33[医药卫生—眼科]